Sharma Lab
Laboratory of intermediary metabolism and metabolic imaging
Gaurav Sharma, Ph.D., MBA, is an Assistant Professor in the Department of Cardiovascular and Thoracic Surgery at UT Southwestern Medical Center. He earned a B.Sc. in biological sciences, an M.Sc. in biotechnology, followed by a Ph.D. in biomedical sciences. He also earned an MBA from the Quantic School of Business and Technology.
The Sharma Lab team has developed an interpretable machine learning-based time-to-event risk scoring tool for lung transplant patients, leveraging UNOS data to predict post-transplant survival at 1, 5, and 10 years. This tool enhances risk stratification and supports personalized clinical decision-making by incorporating key recipient, donor, and transplant-related variables.
Learn more about the Lung Transplant Outcome ToolThe Sharma lab is interested in investigating intermediary metabolism utilizing carbon-13 stable isotope tracers in conjunction with magnetic resonance spectroscopy (MRS), magnetic resonance imaging (MRI), and mass spectrometry (MS). The ongoing projects include:
This study demonstrates that biomarkers of mitochondrial and cytosolic redox may be detected simultaneously in functioning tissues using co-polarized [1-13C]pyruvate and [1,3-13C2]AcAc and 13C MRS and that changes in mitochondrial redox may precede changes in cytosolic redox.
Metabolic remodeling precedes most alterations during cardiac hypertrophic growth under hemodynamic stress. The elevation of glucose utilization has been recognized as a hallmark of metabolic remodeling. However, its role in cardiac hypertrophic growth and heart failure in response to pressure overload remains to be fully illustrated. Here, we aimed to dissect the role of cardiac PKM1 (pyruvate kinase muscle isozyme 1) in glucose metabolic regulation and cardiac response under pressure overload.
In this study, we used hyperpolarized (HP) 13C-magnetic resonance spectroscopy to study the impact of a PDK2/PDK4 double knockout (DKO) on pyruvate metabolism in perfused livers from lean and diet-induced obese (DIO) mice and validated the HP observations with high-resolution 13C-nuclear magnetic resonance (NMR) spectroscopy of tissue extracts and steady-state isotopomer analyses.
In this study, we examined the conversion of hyperpolarized (HP) 13C-acetoacetate (AcAc) to 13C-β-hydroxybutyrate (β-HB) as a potential imaging biomarker for mitochondrial redox and dysfunction in perfused rat hearts.
Glycerol as a precursor for hepatic de novo glutathione synthesis in human liver.
Jin ES, Malloy CR, Sharma G, Finn E, Fuller KNZ, Reyes YG, Lovell MA, Derderian SC, Schoen JA, Inge TH, Cree MG, 2023 Jul Redox Biol 63 102749Oxidation of hyperpolarized [1-13 C]pyruvate in isolated rat kidneys.
Sharma G, Maptue N, Rahim M, Mijes MLT, Hever T, Wen X, Funk AM, Malloy CR, Young JD, Khemtong C, 2022 Oct NMR Biomed e4857PKM1 Exerts Critical Roles in Cardiac Remodeling Under Pressure Overload in the Heart.
Li Q, Li C, Elnwasany A, Sharma G, An YA, Zhang G, Elhelaly WM, Lin J, Gong Y, Chen G, Wang M, Zhao S, Dai C, Smart CD, Liu J, Luo X, Deng Y, Tan L, Lv SJ, Davidson SM, Locasale JW, Lorenzi PL, Malloy CR, Gillette TG, Vander Heiden MG, Scherer PE, Szweda LI, Fu G, Wang ZV, 2021 08 Circulation 9 144 712-727MOXI Is a Mitochondrial Micropeptide That Enhances Fatty Acid ?-Oxidation.
Makarewich CA, Baskin KK, Munir AZ, Bezprozvannaya S, Sharma G, Khemtong C, Shah AM, McAnally JR, Malloy CR, Szweda LI, Bassel-Duby R, Olson EN 2018 Jun Cell Rep 13 23 3701-3709Lactate Dehydrogenase A Governs Cardiac Hypertrophic Growth in Response to Hemodynamic Stress.
Dai C, Li Q, May HI, Li C, Zhang G, Sharma G, Sherry AD, Malloy CR, Khemtong C, Zhang Y, Deng Y, Gillette TG, Xu J, Scadden DT, Wang ZV, 2020 Sep Cell Rep 9 32 108087Mitochondrial Substrate Utilization Regulates Cardiomyocyte Cell Cycle Progression.
Cardoso AC, Lam NT, Savla JJ, Nakada Y, Pereira AHM, Elnwasany A, Menendez-Montes I, Ensley EL, Petric UB, Sharma G, Sherry AD, Malloy CR, Khemtong C, Kinter MT, Tan WLW, Anene-Nzelu CG, Foo RS, Nguyen NUN, Li S, Ahmed MS, Elhelaly WM, Abdisalaam S, Asaithamby A, Xing C, Kanchwala M, Vale G, Eckert KM, Mitsche MA, McDonald JG, Hill JA, Huang L, Shaul PW, Szweda LI, Sadek HA, 2020 Feb Nat Metab 2 2 167-178Probing carbohydrate metabolism using hyperpolarized 13 C-labeled molecules.
Singh J, Suh EH, Sharma G, Khemtong C, Sherry AD, Kovacs Z, 2018 Nov NMR Biomed e4018Role of Moringa oleifera in regulation of diabetes-induced oxidative stress.
Jaiswal D, Rai PK, Mehta S, Chatterji S, Shukla S, Rai DK, Sharma G, Sharma B, Khair S, Watal G, 2013 Jun Asian Pac J Trop Med 6 6 426-32Email address: gaurav.sharma@utsouthwestern.edu
Phone Number: 214-645-7745
Fax Number: 214-645-7701
Mailing Address
UT Southwestern Medical Center
Department of Cardiovascular and Thoracic Surgery
5323 Harry Hines Blvd.
Dallas, TX 75390-8879