Our research takes advantage of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), a technology that we have extensively modified over the years to give us a suite of methods for comprehensive analysis of glycosylation processes of the ER. These innovations have led to many exciting collaborations with investigators both at UT Southwestern and around the world, involving biological samples from various animals, parasites, plants, and bacteria. Here is a partial list of labs that have recently collaborated with us to use FACE, with PubMed links to the Resulting publication that resulted:
- Joseph Contessa, M.D., Ph.D., Yale Cancer Center
High-throughput screening identifies aclacinomycin as a radiosensitizer of EGFR-mutant non-small cell lung cancer. Transl Oncol. 2013 Jun 1;6(3):382-91.
- Joseph Hill, M.D., Ph.D., UT Southwestern
Spliced X-box binding protein 1 couples the unfolded protein response to the hexosamine biosynthetic pathway. Cell. 2014 Mar 13;156(6):1179-92
- Theodore Lampidis, Ph.D., University of Miami
Increased sensitivity to glucose starvation correlates with the downregulation of glycogen phosphorylase isoform PYGB in tumor cell lines resistant to 2-deoxy-D-glucose. Cancer Chemother Pharmacol. 2014 Feb;73(2):349-61.
- Anant Menon, Ph.D., Cornell University
Peter Bütikofer, Ph.D., University of Bern
Glycoprotein biosynthesis in a eukaryote lacking the membrane protein Rft1. J Biol Chem. 2013 Jul 12;288(28):20616-23.
- Philipp Scherer, Ph.D., UT Southwestern
The Xbp1s/GalE axis links ER stress to postprandial hepatic metabolism. J Clin Invest. 2013 Jan 2;123(1):455-68.
- Richard Steet, Ph.D. and Heather Steet, Ph.D., University of Georgia
A zebrafish model of PMM2-CDG reveals altered neurogenesis and a substrate-accumulation mechanism for N-linked glycosylation deficiency. Mol Biol Cell. 2012 Nov;23(21):4175-87.
- Ningguo Gao, Justin Holmes, and Mark Lehrman, Ph.D., UT Southwestern
Letter to the Glycoforum: Improved protocols for preparing lipid-linked and related saccharides for Fluorophore-Assisted Carbohydrate Electrophoresis (FACE). Glycobiology. 2013 Oct;23(10):1111.