Selective Polyprotein Processing Determines Norovirus Sensitivity to Trim7.
Sullender ME, Pierce LR, Annaswamy Srinivas M, Crockett SL, Dunlap BF, Rodgers R, Schriefer LA, Kennedy EA, Stewart BM, Doench JG, Baldridge MT, Orchard RC, 2022 Aug J Virol e0070722Identification of anti-norovirus genes in human cells using genome-wide CRISPR activation screening.
Orchard RC, Sullender ME, Dunlap BF, Balce DR, Doench JG, Virgin HW 2018 Oct J. Virol.
Orchard RC, Wilen CB, Virgin HW
2018 Aug
Nat Microbiol
Tropism for tuft cells determines immune promotion of norovirus pathogenesis.
Wilen CB, Lee S, Hsieh LL, Orchard RC, Desai C, Hykes BL, McAllaster MR, Balce DR, Feehley T, Brestoff JR, Hickey CA, Yokoyama CC, Wang YT, MacDuff DA, Kreamalmayer D, Howitt MR, Neil JA, Cadwell K, Allen PM, Handley SA, van Lookeren Campagne M, Baldridge MT, Virgin HW 2018 Apr Science 6385 360 204-208Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor.
Nelson CA, Wilen CB, Dai YN, Orchard RC, Kim AS, Stegeman RA, Hsieh LL, Smith TJ, Virgin HW, Fremont DH 2018 09 Proc. Natl. Acad. Sci. U.S.A. 39 115 E9201-E9210Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.
Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE 2016 Feb Nat. Biotechnol. 2 34 184-191Discovery of a proteinaceous cellular receptor for a norovirus.
Orchard RC, Wilen CB, Doench JG, Baldridge MT, McCune BT, Lee YC, Lee S, Pruett-Miller SM, Nelson CA, Fremont DH, Virgin HW 2016 08 Science 6302 353 933-6Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.
Orchard RC, Kittisopikul M, Altschuler SJ, Wu LF, Süel GM, Alto NM 2012 Feb Cell 4 148 803-15Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis.
Ortega-Gonzalez P, Taylor G, Jangra RK, Tenorio R, Fernandez de Castro I, Mainou BA, Orchard RC, Wilen CB, Brigleb PH, Sojati J, Chandran K, Sachse M, Risco C, Dermody TS, 2022 Mar PLoS Pathog 3 18 e1010322UFMylation inhibits the proinflammatory capacity of interferon-?-activated macrophages.
Balce DR, Wang YT, McAllaster MR, Dunlap BF, Orvedahl A, Hykes BL, Droit L, Handley SA, Wilen CB, Doench JG, Orchard RC, Stallings CL, Virgin HW, 2021 Jan Proc Natl Acad Sci U S A 1 118
Urbanek K, Sutherland DM, Orchard RC, Wilen CB, Knowlton JJ, Aravamudhan P, Taylor GM, Virgin HW, Dermody TS,
2020 Oct
J Virol
Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection.
Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB, 2020 Oct CellCD300lf conditional knockout mouse reveals strain-specific cellular tropism for murine norovirus.
Graziano VR, Alfajaro MM, Schmitz CO, Filler RB, Strine MS, Wei J, Hsieh LL, Baldridge MT, Nice TJ, Lee S, Orchard RC, Wilen CB, 2020 Nov J VirolGenome-wide CRISPR screen reveals host genes that regulate SARS-CoV-2 infection.
Wei J, Alfajaro MM, Hanna RE, DeWeirdt PC, Strine MS, Lu-Culligan WJ, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Gasque V, de Miguel F, Chen H, Oguntuyo K, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach B, Politi K, van Dijk D, Simon MD, Yan Q, Doench JG, Wilen CB, 2020 Jun bioRxiv
Wang YT, Zaitsev K, Lu Q, Li S, Schaiff WT, Kim KW, Droit L, Wilen CB, Desai C, Balce DR, Orchard RC, Orvedahl A, Park S, Kreamalmeyer D, Handley SA, Pfeifer JD, Baldridge MT, Artyomov MN, Stallings CL, Virgin HW,
2020 Feb
Nat Microbiol
2
5
272-281
Furlong K, Biering SB, Choi J, Wilen CB, Orchard RC, Wobus CE, Nelson CA, Fremont DH, Baldridge MT, Randall G, Hwang S,
2020 08
J Virol
17
94
CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus.
Graziano VR, Walker FC, Kennedy EA, Wei J, Ettayebi K, Strine MS, Filler RB, Hassan E, Hsieh LL, Kim AS, Kolawole AO, Wobus CE, Lindesmith LC, Baric RS, Estes MK, Orchard RC, Baldridge MT, Wilen CB, 2020 04 PLoS Pathog 4 16 e1008242A Secreted Viral Nonstructural Protein Determines Intestinal Norovirus Pathogenesis.
Lee S, Liu H, Wilen CB, Sychev ZE, Desai C, Hykes BL, Orchard RC, McCune BT, Kim KW, Nice TJ, Handley SA, Baldridge MT, Amarasinghe GK, Virgin HW, 2019 May Cell Host Microbe
Orvedahl A, McAllaster MR, Sansone A, Dunlap BF, Desai C, Wang YT, Balce DR, Luke CJ, Lee S, Orchard RC, Artyomov MN, Handley SA, Doench JG, Silverman GA, Virgin HW,
2019 Jul
Proc. Natl. Acad. Sci. U.S.A.
Lee S, Wilen CB, Orvedahl A, McCune BT, Kim KW, Orchard RC, Peterson ST, Nice TJ, Baldridge MT, Virgin HW
2017 Oct
Cell Host Microbe
4
22
449-459.e4
Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases.
Biering SB, Choi J, Halstrom RA, Brown HM, Beatty WL, Lee S, McCune BT, Dominici E, Williams LE, Orchard RC, Wilen CB, Yamamoto M, Coers J, Taylor GA, Hwang S 2017 Jul Cell Host Microbe 1 22 74-85.e7
Weigele BA, Orchard RC, Jimenez A, Cox GW, Alto NM
2017 09
Nat Commun
1
8
532
Clec16a is Critical for Autolysosome Function and Purkinje Cell Survival.
Redmann V, Lamb CA, Hwang S, Orchard RC, Kim S, Razi M, Milam A, Park S, Yokoyama CC, Kambal A, Kreamalmeyer D, Bosch MK, Xiao M, Green K, Kim J, Pruett-Miller SM, Ornitz DM, Allen PM, Beatty WL, Schmidt RE, DiAntonio A, Tooze SA, Virgin HW 2016 Mar Sci Rep 6 23326Noncanonical autophagy inhibits the autoinflammatory, lupus-like response to dying cells.
Martinez J, Cunha LD, Park S, Yang M, Lu Q, Orchard R, Li QZ, Yan M, Janke L, Guy C, Linkermann A, Virgin HW, Green DR 2016 Apr Nature
Selleck EM, Orchard RC, Lassen KG, Beatty WL, Xavier RJ, Levine B, Virgin HW, Sibley LD
2015 Sep
MBio
5
6
e01157-15
Orchard RC, Alto NM
2012 Jan
Cell. Microbiol.
1
14
10-8
The assembly of a GTPase-kinase signalling complex by a bacterial catalytic scaffold.
Selyunin AS, Sutton SE, Weigele BA, Reddick LE, Orchard RC, Bresson SM, Tomchick DR, Alto NM 2011 Jan Nature 7328 469 107-11
Summer EJ, Liu M, Gill JJ, Grant M, Chan-Cortes TN, Ferguson L, Janes C, Lange K, Bertoli M, Moore C, Orchard RC, Cohen ND, Young R
2011 Jan
Appl. Environ. Microbiol.
2
77
669-83
Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics.
Huang Z, Sutton SE, Wallenfang AJ, Orchard RC, Wu X, Feng Y, Chai J, Alto NM 2009 Aug Nat. Struct. Mol. Biol. 8 16 853-60