July 2019 Genes & Development cover

Featured Publication

July 2019 Genes & Development cover. 

See PubMed for full list of publications.

Recent and featured publications:

Mender, I., Siteni, S., Barron, S., Flusche, A.M., Kubota, N., Yu, C., Cornelius, C., Tedone, E., Maziveyi, M., Grichuk, A., Venkateswaran, N., Conacci Sorrell, M., Hoshida, Y., Kang, R., Tang, D., Gryaznov, S., Shay, J.W.. Activating an adaptive immune response with a telomerase-mediated telomere targeting therapeutic in hepatocellular carcinoma, Mol. Cancer Therapeutics, MCT-23-0039, 2023.

Yu, S., Wei, S., Savani, M., Lin, X., Mender, I., Siteni, S., Vasilopulos, T, Reitman, Z., Ku, Y., Wu, d., Liu, H. Tian, M., Chen, Y., Labrie, M., Charbonneau,C., Sugarman, E. Bowie, M.,Seethalakshmi, H. Waitkus, M., Jiang, W., McLendon, R. Pan, E. Kharraw, M. Walsh, K.M., Lu, Y., Wu, K., Shay, J.W., Abdullah, K, Zhang, G., Ashley, D. A modified nucleoside 6-thio-2′-deoxyguanosine exhibits antitumor activity in gliomas. Clin Cancer Res (cover image) November 1 2021 doi:10.1158/1078-0432.CCR-21-0374

Mender, I., Anli Zhang, A. Zhenhua Ren, Z., Han, C., Deng, Y., Siteni, S., Li, H., Zhu, J., Vemula, A., Shay, J.W., and Fu,Y-X. Telomere stress potentiates STING-dependent anti-tumor immunity. Cancer Cell, 38:1-12, Sept 14, 2020 (AOP http; PMC7494563

Sayed ME, Cheng A, Yadav GP, Ludlow AT, Shay JW, Wright WE, Jiang QX. Catalysis-dependent inactivation of human telomerase and its reactivation by intracellular telomerase-activating factors (iTAFs). J Biol Chem. 2019 Jun 11. pii: jbc.RA118.007234. doi: 10.1074/jbc.RA118.007234. [Epub ahead of print] PMID: 31186347

Min J, Wright WE, Shay JW. Clustered telomeres in phase-separated nuclear condensates engage mitotic DNA synthesis through BLM and RAD52. Genes Dev. 2019 Jul 1;33(13-14):814-827. doi: 10.1101/gad.324905.119. Epub 2019 Jun 6. PMID: 31171703

Shay JW, Wright WE. Telomeres and telomerase: three decades of progress. Nat Rev Genet. 2019 May;20(5):299-309. doi: 10.1038/s41576-019-0099-1. Review. PMID: 30760854

Tedone E, Huang E, O'Hara R, Batten K, Ludlow AT, Lai TP, Arosio B, Mari D, Wright WE, Shay JW.Telomere length and telomerase activity in T cells are biomarkers of high-performing centenarians. Aging Cell. 2019 Feb;18(1):e12859. doi: 10.1111/acel.12859. Epub 2018 Nov 28. PMID: 30488553

Mender I, LaRanger R, Luitel K, Peyton M, Girard L, Lai TP, Batten K, Cornelius C, Dalvi MP, Ramirez M, Du W, Wu LF, Altschuler SJ, Brekken R, Martinez ED, Minna JD, Wright WE, Shay JW. Telomerase-Mediated Strategy for Overcoming Non-Small Cell Lung Cancer Targeted Therapy and Chemotherapy Resistance. Neoplasia. 2018 Aug;20(8):826-837. doi: 10.1016/j.neo.2018.06.002. Epub 2018 Jul 6. PMID: 30015158

Ludlow AT, Shelton D, Wright WE, Shay JW. ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity. Methods Mol Biol. 2018;1768:513-529. doi: 10.1007/978-1-4939-7778-9_29. PMID: 29717462

Lai TP, Zhang N, Noh J, Mender I, Tedone E, Huang E, Wright WE, Danuser G, Shay JW. A method for measuring the distributions of the shortest telomeres in cells and tissues. Nature Communications. In press. 2017.

Shay JW. New insights into melanoma development. Science. 2017 Sep 29;357(6358):1358-1359. doi: 10.1126/science.aao6963. Epub 2017 Sep 28. PMID: 28963245

Kim W, Ludlow AT, Min J, Robin JD, Stadler G, Mender I, Lai TP, Zhang N, Wright WE, Shay JW.Regulation of the Human Telomerase Gene TERT by Telomere Position Effect-Over Long Distances (TPE-OLD): Implications for Aging and Cancer. PLoS Biol. 2016 Dec 15;14(12):e2000016. doi:10.1371/journal.pbio.2000016. eCollection 2016 Dec. PMID: 27977688

Min J, Shay JW. TERT Promoter Mutations Enhance Telomerase Activation by Long-Range Chromatin Interactions. Cancer Discov. 2016 Nov;6(11):1212-1214. PMID: 27807101

Zhang L, Theodoropoulos PC, Eskiocak U, Wang W, Moon YA, Posner B, Williams NS, Wright WE, Kim SB, Nijhawan D, De Brabander JK, Shay JW. Selective targeting of mutant adenomatous polyposis coli (APC) in colorectal cancer. Sci Transl Med. 2016 Oct 19;8(361):361ra140. PMID: 27798265

Shay JW. Role of Telomeres and Telomerase in Aging and Cancer. Cancer Discov. 2016 Jun;6(6):584-93. doi: 10.1158/2159-8290.CD-16-0062. Epub 2016 Mar 30. Review. PMID: 27029895

Pandita RK, Chow TT, Udayakumar D, Bain AL, Cubeddu L, Hunt CR, Shi W, Horikoshi N, Zhao Y, Wright WE, Khanna KK, Shay JW, Pandita TK. Single-strand DNA binding protein SSB1 acilitates TERT recruitment to telomeres and maintains telomere G-overhangs. Cancer Res. 2015 Jan 14. pii: canres.2289.2014. [Epub ahead of print] PubMed PMID: 25589350. 

Mender I, Gryaznov S, Dikmen ZG, Wright WE, Shay JW. Induction of telomere dysfunction mediated by the telomerase substrate precursor 6-thio-2'-deoxyguanosine. Cancer Discov. 2015 Jan;5(1):82-95. doi: 10.1158/2159-8290.CD-14-0609. Epub 2014 Dec 16. PubMed PMID: 25516420; PubMed Central PMCID: PMC4293221. 

Robin JD, Ludlow AT, Batten K, Magdinier F, Stadler G, Wagner KR, Shay JW, Wright WE. Telomere position effect: regulation of gene expression with progressive telomere shortening over long distances. Genes Dev. 2014 Nov 15;28(22):2464-76. doi: 10.1101/gad.251041.114. PubMed PMID: 25403178; PubMed Central PMCID: PMC4233240.

Wong MS, Wright WE, Shay JW. Alternative splicing regulation of telomerase: a new paradigm? Trends Genet. 2014 Oct;30(10):430-8. doi: 10.1016/j.tig.2014.07.006. Epub 2014 Aug 26. PubMed PMID: 25172021; PubMed Central PMCID: PMC4190675.

Ludlow AT, Robin JD, Sayed M, Litterst CM, Shelton DN, Shay JW, Wright WE. Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution. Nucleic Acids Res. 2014 Jul;42(13):e104. doi: 10.1093/nar/gku439. Epub 2014 May 26. PubMed PMID: 24861623; PubMed Central PMCID: PMC4117742.

Holohan B, Wright WE, Shay JW. Cell biology of disease: Telomeropathies: an emerging spectrum disorder. J Cell Biol. 2014 May 12;205(3):289-99. doi: 10.1083/jcb.201401012. Review. PubMed PMID: 24821837; PubMed Central PMCID: PMC4018777.

Singel SM, Batten K, Cornelius C, Jia G, Fasciani G, Barron SL, Wright WE, Shay JW. Receptor-interacting protein kinase 2 promotes triple-negative breast cancer cell migration and invasion via activation of nuclear factor-kappaB and c-Jun N-terminal kinase pathways. Breast Cancer Res. 2014 Mar 19;16(2):R28. doi: 10.1186/bcr3629. PubMed PMID: 24642040; PubMed Central PMCID: PMC4053227.

Delgado O, Batten KG, Richardson JA, Xie XJ, Gazdar AF, Kaisani AA, Girard L, Behrens C, Suraokar M, Fasciani G, Wright WE, Story MD, Wistuba II, Minna JD, Shay JW. Radiation-enhanced lung cancer progression in a transgenic mouse model of lung cancer is predictive of outcomes in human lung and breast cancer. Clin Cancer Res. 2014 Mar 15;20(6):1610-22. doi: 10.1158/1078-0432.CCR-13-2589. Epub 2014 Jan 31. PubMed PMID: 24486591; PubMed Central PMCID: PMC3961755.

Kaisani A, Delgado O, Fasciani G, Kim SB, Wright WE, Minna JD, Shay JW. Branching morphogenesis of immortalized human bronchial epithelial cells in three-dimensional culture. Differentiation. 2014 Mar-Apr;87(3-4):119-26. doi: 10.1016/j.diff.2014.02.003. Epub 2014 May 13. PubMed PMID: 24830354; PubMed Central PMCID: PMC4112006.

Singel SM, Cornelius C, Zaganjor E, Batten K, Sarode VR, Buckley DL, Peng Y, John GB, Li HC, Sadeghi N, Wright WE, Lum L, Corson TW, Shay JW. KIF14 promotes AKT phosphorylation and contributes to chemoresistance in triple-negative breast cancer. Neoplasia. 2014 Mar;16(3):247-56, 256.e2. doi: 10.1016/j.neo.2014.03.008. PubMed PMID: 24784001; PubMed Central PMCID: PMC4094827.

Wong MS, Shay JW, Wright WE. Regulation of human telomerase splicing by RNA:RNA pairing. Nat Commun. 2014 Feb 28;5:3306. doi: 10.1038/ncomms4306. PubMed PMID: 24577044; PubMed Central PMCID: PMC3948165.

El-Ashmawy M, Delgado O, Cardentey A, Wright WE, Shay JW. CDDO-Me Protects Normal Lung and Breast Epithelial Cells but Not Cancer Cells from Radiation. PLoS One. 2014 Dec 23;9(12):e115600. doi: 10.1371/journal.pone.0115600. eCollection 2014. PubMed PMID: 25536195; PubMed Central PMCID: PMC4275221.

Kim SB, Ly P, Kaisani A, Zhang L, Wright WE, Shay JW. Mitigation of radiation-induced damage by targeting EGFR in noncancerous human epithelial cells. Radiat Res. 2013 Sep;180(3):259-67. doi: 10.1667/RR3371.1. Epub 2013 Aug 6. PubMed PMID: 23919312.

Ly P, Kim SB, Kaisani AA, Marian G, Wright WE, Shay JW. Aneuploid human colonic epithelial cells are sensitive to AICAR-induced growth inhibition through EGFR degradation. Oncogene. 2013 Jun 27;32(26):3139-46. doi: 10.1038/onc.2012.339. Epub 2012 Aug 13. PubMed PMID: 22890317; PubMed Central PMCID: PMC3983693.

Stadler G, Rahimov F, King OD, Chen JC, Robin JD, Wagner KR, Shay JW, Emerson CP Jr, Wright WE. Telomere position effect regulates DUX4 in human facioscapulohumeral muscular dystrophy. Nat Struct Mol Biol. 2013 Jun;20(6):671-8. doi: 10.1038/nsmb.2571. Epub 2013 May 5. PubMed PMID: 23644600; PubMed Central PMCID: PMC3711615.

Wong MS, Chen L, Foster C, Kainthla R, Shay JW, Wright WE. Regulation of telomerase alternative splicing: a target for chemotherapy. Cell Rep. 2013 Apr 25;3(4):1028-35. doi: 10.1016/j.celrep.2013.03.011. Epub 2013 Apr 4. PubMed PMID: 23562158; PubMed Central PMCID: PMC3640656.

Singel SM, Cornelius C, Batten K, Fasciani G, Wright WE, Lum L, Shay JW. A targeted RNAi screen of the breast cancer genome identifies KIF14 and TLN1 as genes that modulate docetaxel chemosensitivity in triple-negative breast cancer. Clin Cancer Res. 2013 Apr 15;19(8):2061-70. doi: 10.1158/1078-0432.CCR-13-0082. Epub 2013 Mar 11. PubMed PMID: 23479679. 

Zhang L, Komurov K, Wright WE, Shay JW. Identification of novel driver tumor suppressors through functional interrogation of putative passenger mutations in colorectal cancer. Int J Cancer. 2013 Feb 1;132(3):732-7. doi: 10.1002/ijc.27705. Epub 2012 Jul 21. PubMed PMID: 22753261.

Stadler G, King OD, Robin JD, Shay JW, Wright WE. Facioscapulohumeral muscular dystrophy: Are telomeres the end of the story? Rare Dis. 2013 Aug 14;1:e26142. doi: 10.4161/rdis.26142. eCollection 2013. PubMed PMID: 25003004; PubMed Central PMCID: PMC3927483.

Ly P, Eskiocak U, Parker CR, Harris KJ, Wright WE, Shay JW. RNAi screening of the human colorectal cancer genome identifies multifunctional tumor suppressors regulating epithelial cell invasion. Cell Res. 2012 Nov;22(11):1605-8. doi: 10.1038/cr.2012.140. Epub 2012 Oct 9. PubMed PMID: 23044803; PubMed Central PMCID: PMC3494398.

Kim SB, Pandita RK, Eskiocak U, Ly P, Kaisani A, Kumar R, Cornelius C, Wright WE, Pandita TK, Shay JW. Targeting of Nrf2 induces DNA damage signaling and protects colonic epithelial cells from ionizing radiation. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):E2949-55. doi: 10.1073/pnas.1207718109. Epub 2012 Oct 8. PubMed PMID: 23045680; PubMed Central PMCID: PMC3491493.

Shay JW, Reddel RR, Wright WE. Cancer. Cancer and telomeres--an ALTernative to telomerase. Science. 2012 Jun 15;336(6087):1388-90. doi: 10.1126/science.1222394. PubMed PMID: 22700908.

Chow TT, Zhao Y, Mak SS, Shay JW, Wright WE. Early and late steps in telomere overhang processing in normal human cells: the position of the final RNA primer drives telomere shortening. Genes Dev. 2012 Jun 1;26(11):1167-78. doi: 10.1101/gad.187211.112. PubMed PMID: 22661228; PubMed Central PMCID: PMC3371406.

Hoshiyama H, Tang J, Batten K, Xiao G, Rouillard JM, Shay JW, Xie Y, Wright WE. Development of methods for quantitative comparison of pooled shRNAs by mass sequencing. J Biomol Screen. 2012 Feb;17(2):258-65. doi: 10.1177/1087057111423101. Epub 2011 Sep 28. PubMed PMID: 21956173.

Buseman CM, Wright WE, Shay JW. Is telomerase a viable target in cancer? Mutat Res. 2012 Feb 1;730(1-2):90-7. doi: 10.1016/j.mrfmmm.2011.07.006. Epub 2011 Jul 23. Review. PubMed PMID: 21802433; PubMed Central PMCID: PMC3375693. 

Kim J, Eskiocak U, Stadler G, Lou Z, Kuro-o M, Shay JW, Wright WE. Short hairpin RNA screen indicates that Klotho beta/FGF19 protein overcomes stasis in human colonic epithelial cells. J Biol Chem. 2011 Dec 16;286(50):43294-300. doi: 10.1074/jbc.M111.267641. Epub 2011 Oct 21. PubMed PMID: 22020932; PubMed Central PMCID: PMC3234855.

Shay JW, Wright WE. Role of telomeres and telomerase in cancer. Semin Cancer Biol. 2011 Dec;21(6):349-53. doi: 10.1016/j.semcancer.2011.10.001. Epub 2011 Oct 17. Review. PubMed PMID: 22015685; PubMed Central PMCID: PMC3370415.

Gomes NM, Ryder OA, Houck ML, Charter SJ, Walker W, Forsyth NR, Austad SN, Venditti C, Pagel M, Shay JW, Wright WE. Comparative biology of mammalian telomeres: hypotheses on ancestral states and the roles of telomeres in longevity determination. Aging Cell. 2011 Oct;10(5):761-8. doi: 10.1111/j.1474-9726.2011.00718.x. Epub 2011 Jun 1. PubMed PMID: 21518243; PubMed Central PMCID: PMC3387546.

Eskiocak U, Kim SB, Ly P, Roig AI, Biglione S, Komurov K, Cornelius C, Wright WE, White MA, Shay JW. Functional parsing of driver mutations in the colorectal cancer genome reveals numerous suppressors of anchorage-independent growth. Cancer Res. 2011 Jul 1;71(13):4359-65. doi: 10.1158/0008-5472.CAN-11-0794. Epub 2011 Apr 28. PubMed PMID: 21527559; PubMed Central PMCID: PMC3326261.

Ouellette MM, Wright WE, Shay JW. Targeting telomerase-expressing cancer cells. J Cell Mol Med. 2011 Jul;15(7):1433-42. doi: 10.1111/j.1582-4934.2011.01279.x. Review. PubMed PMID: 21332640; PubMed Central PMCID: PMC3370414.

Zhao Y, Abreu E, Kim J, Stadler G, Eskiocak U, Terns MP, Terns RM, Shay JW, Wright WE. Processive and distributive extension of human telomeres by telomerase under homeostatic and nonequilibrium conditions. Mol Cell. 2011 May 6;42(3):297-307. doi: 10.1016/j.molcel.2011.03.020. PubMed PMID: 21549308; PubMed Central PMCID: PMC3108241.

Ly P, Eskiocak U, Kim SB, Roig AI, Hight SK, Lulla DR, Zou YS, Batten K, Wright WE, Shay JW. Characterization of aneuploid populations with trisomy 7 and 20 derived from diploid human colonic epithelial cells. Neoplasia. 2011 Apr;13(4):348-57. PubMed PMID: 21472139; PubMed Central PMCID: PMC3071083. 

Stadler G, Chen JC, Wagner K, Robin JD, Shay JW, Emerson CP Jr, Wright WE. Establishment of clonal myogenic cell lines from severely affected dystrophic muscles - CDK4 maintains the myogenic population. Skelet Muscle. 2011 Mar 8;1(1):12. doi: 10.1186/2044-5040-1-12. PubMed PMID: 21798090; PubMed Central PMCID: PMC3156635.

Zhao Y, Shay JW, Wright WE. Telomere terminal G/C strand synthesis: measuring telomerase action and C-rich fill-in. Methods Mol Biol. 2011;735:63-75. doi: 10.1007/978-1-61779-092-8_7. PubMed PMID: 21461812; PubMed Central PMCID: PMC3528099. 

Zhao Y, Shay JW, Wright WE. Telomere G-overhang length measurement method 1: the DSN method. Methods Mol Biol. 2011;735:47-54. doi: 10.1007/978-1-61779-092-8_5. PubMed PMID: 21461810; PubMed Central PMCID: PMC3528100.

Delgado O, Kaisani AA, Spinola M, Xie XJ, Batten KG, Minna JD, Wright WE, Shay JW. Multipotent capacity of immortalized human bronchial epithelial cells. PLoS One. 2011;6(7):e22023. doi: 10.1371/journal.pone.0022023. Epub 2011 Jul 7. PubMed PMID: 21760947; PubMed Central PMCID: PMC3131301.

Joseph I, Tressler R, Bassett E, Harley C, Buseman CM, Pattamatta P, Wright WE, Shay JW, Go NF. The telomerase inhibitor imetelstat depletes cancer stem cells in breast and pancreatic cancer cell lines. Cancer Res. 2010 Nov 15;70(22):9494-504. doi: 10.1158/0008-5472.CAN-10-0233. Epub 2010 Nov 9. PubMed PMID: 21062983.

Gomes NM, Shay JW, Wright WE. Telomere biology in Metazoa. FEBS Lett. 2010 Sep 10;584(17):3741-51. doi: 10.1016/j.febslet.2010.07.031. Epub 2010 Jul 23. Review. PubMed PMID: 20655915; PubMed Central PMCID: PMC2928394.

Shay JW, Wright WE. Telomeres and telomerase in normal and cancer stem cells. FEBS Lett. 2010 Sep 10;584(17):3819-25. doi: 10.1016/j.febslet.2010.05.026. Epub 2010 May 21. Review. PubMed PMID: 20493857; PubMed Central PMCID: PMC3370416.

Marian CO, Wright WE, Shay JW. The effects of telomerase inhibition on prostate tumor-initiating cells. Int J Cancer. 2010 Jul 15;127(2):321-31. doi: 10.1002/ijc.25043. PubMed PMID: 19908230.

Eskiocak U, Kim SB, Roig AI, Kitten E, Batten K, Cornelius C, Zou YS, Wright WE, Shay JW. CDDO-Me protects against space radiation-induced transformation of human colon epithelial cells. Radiat Res. 2010 Jul;174(1):27-36. doi:10.1667/RR2155.1. PubMed PMID: 20681796.

Roig AI, Eskiocak U, Hight SK, Kim SB, Delgado O, Souza RF, Spechler SJ, Wright WE, Shay JW. Immortalized epithelial cells derived from human colon biopsies express stem cell markers and differentiate in vitro. Gastroenterology. 2010 Mar;138(3):1012-21.e1-5. doi: 10.1053/j.gastro.2009.11.052. Epub 2009 Dec 4. PubMed PMID: 19962984.

Atzmon G, Cho M, Cawthon RM, Budagov T, Katz M, Yang X, Siegel G, Bergman A, Huffman DM, Schechter CB, Wright WE, Shay JW, Barzilai N, Govindaraju DR, Suh Y. Evolution in health and medicine Sackler colloquium: Genetic variation in human telomerase is associated with telomere length in Ashkenazi centenarians. Proc Natl Acad Sci U S A. 2010 Jan 26;107 Suppl 1:1710-7. doi: 10.1073/pnas.0906191106. Epub 2009 Nov 13. PubMed PMID: 19915151; PubMed Central PMCID: PMC2868292. 

Marian CO, Cho SK, McEllin BM, Maher EA, Hatanpaa KJ, Madden CJ, Mickey BE, Wright WE, Shay JW, Bachoo RM. The telomerase antagonist, imetelstat, efficiently targets glioblastoma tumor-initiating cells leading to decreased proliferation and tumor growth. Clin Cancer Res. 2010 Jan 1;16(1):154-63. doi: 10.1158/1078-0432.CCR-09-2850. PubMed PMID: 20048334; PubMed Central PMCID: PMC2883447.

Vaughan MB, Ramirez RD, Andrews CM, Wright WE, Shay JW. H-ras expression in immortalized keratinocytes produces an invasive epithelium in cultured skin equivalents. PLoS One. 2009 Nov 19;4(11):e7908. doi: 10.1371/journal.pone.0007908. PubMed PMID: 19936293; PubMed Central PMCID:PMC2774948. 

Zhao Y, Sfeir AJ, Zou Y, Buseman CM, Chow TT, Shay JW, Wright WE. Telomere extension occurs at most chromosome ends and is uncoupled from fill-in in human cancer cells. Cell. 2009 Aug 7;138(3):463-75. doi: 10.1016/j.cell.2009.05.026. PubMed PMID: 19665970; PubMed Central PMCID: PMC2726829.

Lou Z, Wei J, Riethman H, Baur JA, Voglauer R, Shay JW, Wright WE. Telomere length regulates ISG15 expression in human cells. Aging (Albany NY). 2009 Jul 17;1(7):608-21. PubMed PMID: 20157543; PubMed Central PMCID: PMC2806043.

Zou Y, Misri S, Shay JW, Pandita TK, Wright WE. Altered states of telomeredeprotection and the two-stage mechanism of replicative aging. Mol Cell Biol. 2009 May;29(9):2390-7. doi: 10.1128/MCB.01569-08. Epub 2009 Feb 17. PubMed PMID:19223460; PubMed Central PMCID: PMC2668373. 

Matsuo T, Shay JW, Wright WE, Hiyama E, Shimose S, Kubo T, Sugita T, Yasunaga Y, Ochi M. Telomere-maintenance mechanisms in soft-tissue malignant fibroushistiocytomas. J Bone Joint Surg Am. 2009 Apr;91(4):928-37. doi:10.2106/JBJS.G.01390. PubMed PMID: 19339578.