Publications

Featured Publications

Functional analysis of 3' UTR hairpins supports a two-tiered model for posttranscriptional regulation of MAT2A by METTL16.

Hunter OV, Ruiz JC, Flaherty JN, Conrad NK, 2023 Aug RNA

The PNUTS-PP1 complex acts as an intrinsic barrier to herpesvirus KSHV gene expression and replication.

Devlin AM, Shukla A, Ruiz JC, Barnes SD, Govindan A, Hunter OV, Scarborough AM, D'Orso I, Conrad NK, 2022 Dec Nat Commun 1 13 7447

Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention.

Scarborough AM, Govindan A, Conrad NK, 2022 Methods Mol Biol 2537 263-284

SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A.

Scarborough AM, Flaherty JN, Hunter OV, Liu K, Kumar A, Xing C, Tu BP, Conrad NK, 2021 May Elife 10

Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.

Ruiz JC, Devlin AM, Kim J, Conrad NK, 2020 07 J Virol 14 94

Kaposi's sarcoma-associated herpesvirus ORF57 protein protects viral transcripts from specific nuclear RNA decay pathways by preventing hMTR4 recruitment.

Ruiz JC, Hunter OV, Conrad NK, 2019 02 PLoS Pathog. 2 15 e1007596

Balance between MAT2A intron detention and splicing is determined co-transcriptionally.

Pendleton KE, Park SK, Hunter OV, Bresson SM, Conrad NK 2018 Mar RNA

The U6 snRNA m(6)A Methyltransferase METTL16 Regulates SAM Synthetase Intron Retention.

Pendleton KE, Chen B, Liu K, Hunter OV, Xie Y, Tu BP, Conrad NK 2017 May Cell 5 169 824-835.e14

A Conserved Splicing Silencer Dynamically Regulates O-GlcNAc Transferase Intron Retention and O-GlcNAc Homeostasis.

Park SK, Zhou X, Pendleton KE, Hunter OV, Kohler JJ, O'Donnell KA, Conrad NK 2017 Aug Cell Rep 5 20 1088-1099

Canonical Poly(A) Polymerase Activity Promotes the Decay of a Wide Variety of Mammalian Nuclear RNAs.

Bresson SM, Hunter OV, Hunter AC, Conrad NK 2015 Oct PLoS Genet. 10 11 e1005610

New insights into the expression and functions of the Kaposi's sarcoma-associated herpesvirus long noncoding PAN RNA.

Conrad NK 2015 Jun Virus Res.

Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.

Stubbs SH, Conrad NK 2015 Jan Nucleic Acids Res. 1 43 504-19

HITS-CLIP analysis uncovers a link between the Kaposi's sarcoma-associated herpesvirus ORF57 protein and host pre-mRNA metabolism.

Sei E, Wang T, Hunter OV, Xie Y, Conrad NK 2015 Feb PLoS Pathog. 2 11 e1004652

Analysis of RNA-protein interactions by cell mixing.

Stubbs SH, Conrad NK 2014 Meth. Enzymol. 539 67-80

UV cross-linking of interacting RNA and protein in cultured cells.

Sei E, Conrad NK 2014 Meth. Enzymol. 539 53-66

ChIP-chip analysis suggests functional cooperation between Kaposi's sarcoma-associated herpesvirus ORF57 and K-bZIP.

Hunter OV, Sei E, Richardson RB, Conrad NK 2013 Jan J. Virol.

The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay.

Bresson SM, Conrad NK 2013 PLoS Genet. 10 9 e1003893

Chemical reporters for monitoring RNA synthesis and poly(A) tail dynamics.

Grammel M, Hang H, Conrad NK 2012 May Chembiochem 8 13 1112-5

Viral Factors Reveal a Role for REF/Aly in Nuclear RNA Stability.

Stubbs SH, Hunter OV, Hoover A, Conrad NK 2012 Apr Mol. Cell. Biol. 7 32 1260-70

Delineation of a core RNA element required for Kaposi's sarcoma-associated herpesvirus ORF57 binding and activity.

Sei E, Conrad NK 2011 Oct Virology 2 419 107-16

Kaposi's sarcoma-associated herpesvirus ORF57 protein binds and protects a nuclear noncoding RNA from cellular RNA decay pathways.

Sahin BB, Patel D, Conrad NK 2010 Mar PLoS Pathog. 3 6 e1000799

Posttranscriptional gene regulation in Kaposi's sarcoma-associated herpesvirus.

Conrad NK 2009 Adv. Appl. Microbiol. 68 241-61

Chapter 15. Co-immunoprecipitation techniques for assessing RNA-protein interactions in vivo.

Conrad NK 2008 Meth. Enzymol. 449 317-42

Mutational analysis of a viral RNA element that counteracts rapid RNA decay by interaction with the polyadenylate tail.

Conrad NK, Shu MD, Uyhazi KE, Steitz JA 2007 Jun Proc. Natl. Acad. Sci. U.S.A. 25 104 10412-7

Identification of a rapid mammalian deadenylation-dependent decay pathway and its inhibition by a viral RNA element.

Conrad NK, Mili S, Marshall EL, Shu MD, Steitz JA 2006 Dec Mol. Cell 6 24 943-53

A Kaposi's sarcoma virus RNA element that increases the nuclear abundance of intronless transcripts.

Conrad NK, Steitz JA 2005 May EMBO J. 10 24 1831-41

Influenza Virus NS1 Protein RNA-Interactome Reveals Intron Targeting.

Zhang L, Wang J, Muñoz-Moreno R, Kim M, Sakthivel R, Mo W, Shao D, Anantharaman A, García-Sastre A, Conrad NK, Fontoura BMA 2018 Sep J. Virol.

Structural Basis for Regulation of METTL16, an S-Adenosylmethionine Homeostasis Factor.

Doxtader KA, Wang P, Scarborough AM, Seo D, Conrad NK, Nam Y 2018 Sep Mol. Cell 6 71 1001-1011.e4

Poly(A) tail length regulates PABPC1 expression to tune translation in the heart.

Chorghade S, Seimetz J, Emmons R, Yang J, Bresson SM, Lisio M, Parise G, Conrad NK, Kalsotra A 2017 Jun Elife 6

ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins.

Anantharaman A, Tripathi V, Khan A, Yoon JH, Singh DK, Gholamalamdari O, Guang S, Ohlson J, Wahlstedt H, Öhman M, Jantsch MF, Conrad NK, Ma J, Gorospe M, Prasanth SG, Prasanth KV 2017 Jan Nucleic Acids Res.

The emerging role of triple helices in RNA biology.

Conrad NK 2014 Jan-Feb Wiley Interdiscip Rev RNA 1 5 15-29