In vitro RapGAP activity assay

This is a real-time photometric assay based on (Webb MR et al, 1992, Biochem J.) and (Webb MR, 1992, PNAS)

The detailed descriptions of this method is in this publication:

Pascoe HG*, Wang Y* and Zhang X.
In vitro assay for the Rap GTPase activating protein activity of the purified cytoplasmic domain of plexin.
Methods Mol. Bio. 2017, 1493:107-118.

Cos7 cell collapse assay

This assay, originally developed by the Strittmatter group, provides a qualitative measure for the activity of plexin in cells.