Publications

Featured Publications

CCT and CCT-like Modular Protein Interaction Domains in WNK Signaling.

Taylor CA, Cobb MH, 2021 Jul Mol Pharmacol

WNK1 Enhances Migration and Invasion in Breast Cancer Models.

Jaykumar AB, Jung JU, Parida PK, Dang TT, Wichaidit C, Kannangara AR, Earnest S, Goldsmith EJ, Pearson GW, Malladi S, Cobb MH, 2021 Jul Mol Cancer Ther

The pancreatic ? cell response to secretory demands and adaption to stress

M.A. Kalwat, D. Scheuner, K. Rodrigues Dos Santos, D.L. Eizirik, and M.H. Cobb 2021

a2-Adrenergic Disruption of ? Cell BDNF-TrkB Receptor Tyrosine Kinase Signaling.

Kalwat MA, Huang Z, Binns DD, McGlynn K, Cobb MH, 2020 Front Cell Dev Biol 8 576396

Control of Podocyte and Glomerular Capillary Wall Structure and Elasticity by WNK1 Kinase.

Liu Z, Yoon J, Wichaidit C, Jaykumar AB, Dbouk HA, Embry AE, Liu L, Henderson JM, Chang AN, Cobb MH, Miller RT, 2020 Front Cell Dev Biol 8 618898

Functional divergence caused by mutations in an energetic hotspot in ERK2.

Taylor CA, Cormier KW, Keenan SE, Earnest S, Stippec S, Wichaidit C, Juang YC, Wang J, Shvartsman SY, Goldsmith EJ, Cobb MH, 2019 Jul Proc. Natl. Acad. Sci. U.S.A. 31 116 15514-15523

OSR1 regulates a subset of inward rectifier potassium channels via a binding motif variant.

Taylor CA, An SW, Kankanamalage SG, Stippec S, Earnest S, Trivedi AT, Yang JZ, Mirzaei H, Huang CL, Cobb MH 2018 Mar Proc. Natl. Acad. Sci. U.S.A.

Enteroid Monolayers Reveal an Autonomous WNT and BMP Circuit Controlling Intestinal Epithelial Growth and Organization.

Thorne CA, Chen IW, Sanman LE, Cobb MH, Wu LF, Altschuler SJ 2018 Mar Dev. Cell 5 44 624-633.e4

Differential abundance of CK1a provides selectivity for pharmacological CK1a activators to target WNT-dependent tumors.

Li B, Orton D, Neitzel LR, Astudillo L, Shen C, Long J, Chen X, Kirkbride KC, Doundoulakis T, Guerra ML, Zaias J, Fei DL, Rodriguez-Blanco J, Thorne C, Wang Z, Jin K, Nguyen DM, Sands LR, Marchetti F, Abreu MT, Cobb MH, Capobianco AJ, Lee E, Robbins DJ 2017 Jun Sci Signal 485 10

Amino Acids Regulate mTORC1 by an Obligate Two-Step Mechanism.

Dyachok J, Earnest S, Iturraran EN, Cobb MH, Ross EM 2016 Sep J. Biol. Chem.

Insulin promoter-driven Gaussia luciferase-based insulin secretion biosensor assay for discovery of ?-cell glucose-sensing pathways.

Kalwat MA, Wichaidit C, Nava Garcia AY, McCoy MK, McGlynn K, Hwang IH, MacMillan JB, Posner BA, Cobb MH 2016 Oct ACS Sens 10 1 1208-1212

Phosphorylation or Mutation of the ERK2 Activation Loop Alters Oligonucleotide Binding.

McReynolds AC, Karra AS, Li Y, Lopez ED, Turjanski AG, Dioum E, Lorenz K, Zaganjor E, Stippec S, McGlynn K, Earnest S, Cobb MH 2016 Mar Biochemistry 12 55 1909-17

ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs.

Borromeo MD, Savage TK, Kollipara RK, He M, Augustyn A, Osborne JK, Girard L, Minna JD, Gazdar AF, Cobb MH, Johnson JE 2016 Jul Cell Rep

Isoxazole Alters Metabolites and Gene Expression, Decreasing Proliferation and Promoting a Neuroendocrine Phenotype in ?-Cells.

Kalwat MA, Huang Z, Wichaidit C, McGlynn K, Earnest S, Savoia C, Dioum EM, Schneider JW, Hutchison MR, Cobb MH 2016 Feb ACS Chem. Biol.

Multistep regulation of autophagy by WNK1.

Gallolu Kankanamalage S, Lee AY, Wichaidit C, Lorente-Rodriguez A, Shah AM, Stippec S, Whitehurst AW, Cobb MH 2016 Dec Proc. Natl. Acad. Sci. U.S.A. 50 113 14342-14347

Chemoattractant concentration-dependent tuning of ERK signaling dynamics in migrating neutrophils.

Zhang ER, Liu S, Wu LF, Altschuler SJ, Cobb MH 2016 Dec Sci Signal 458 9 ra122

The domain-swapping switch point in Ste20 protein kinase SPAK.

Taylor CA, Juang YC, Earnest S, Sengupta S, Goldsmith EJ, Cobb MH 2015 Jul Biochemistry

Actions of the protein kinase WNK1 on endothelial cells are differentially mediated by its substrate kinases OSR1 and SPAK.

Dbouk HA, Weil LM, Perera GK, Dellinger MT, Pearson G, Brekken RA, Cobb MH 2014 Nov Proc. Natl. Acad. Sci. U.S.A. 45 111 15999-6004

Menin determines K-RAS proliferative outputs in endocrine cells.

Chamberlain CE, Scheel DW, McGlynn K, Kim H, Miyatsuka T, Wang J, Nguyen V, Zhao S, Mavropoulos A, Abraham AG, O'Neill E, Ku GM, Cobb MH, Martin GR, German MS 2014 Aug J. Clin. Invest.

Muscarinic Control of MIN6 Pancreatic ? Cells Is Enhanced by Impaired Amino Acid Signaling.

Guerra ML, Wauson EM, McGlynn K, Cobb MH 2014 Apr J. Biol. Chem.

Chloride Sensing by WNK1 Involves Inhibition of Autophosphorylation.

Piala AT, Moon TM, Akella R, He H, Cobb MH, Goldsmith EJ 2014 Sci Signal 324 7 ra41

The G Protein-Coupled Taste Receptor T1R1/T1R3 Regulates mTORC1 and Autophagy.

Wauson EM, Zaganjor E, Lee AY, Guerra ML, Ghosh AB, Bookout AL, Chambers CP, Jivan A, McGlynn K, Hutchison MR, Deberardinis RJ, Cobb MH 2012 Sep Mol. Cell 6 47 851-62

Protein kinase WNK3 regulates the neuronal splicing factor Fox-1.

Lee AY, Chen W, Stippec S, Self J, Yang F, Ding X, Chen S, Juang YC, Cobb MH 2012 Oct Proc. Natl. Acad. Sci. U.S.A. 42 109 16841-6

WNK 1 is required for mitosis and abscission.

S-W. Tu, K. Luby-Phelps and M.H. Cobb. 2011 108 1385-1390

Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback.

L. Duan and M.H. Cobb. 2010 107 22314-22319

The Epithelial Sodium Channel (aENaC) Is a Downstream Therapeutic Target of ASCL1 in Pulmonary Neuroendocrine Tumors.

He M, Liu S, Gallolu Kankanamalage S, Borromeo MD, Girard L, Gazdar AF, Minna JD, Johnson JE, Cobb MH 2018 Feb Transl Oncol 2 11 292-299

Assaying Protein Kinase Activity with Radiolabeled ATP.

Karra AS, Stippec S, Cobb MH 2017 May J Vis Exp 123

Correction to Insulin Promoter-Driven Gaussia Luciferase-Based Insulin Secretion Biosensor Assay for Discovery of ?-Cell Glucose-Sensing Pathways.

Kalwat MA, Wichaidit C, Nava Garcia AY, McCoy MK, McGlynn K, Hwang IH, MacMillan JB, Posner BA, Cobb MH 2017 Feb ACS Sens 2 2 316

Hypertension: The Missing WNKs.

Dbouk HA, Huang CL, Cobb MH 2016 Mar Am. J. Physiol. Renal Physiol. ajprenal.00358.2015

Small Cell Lung Cancer: Can recent advances in biology and molecular biology be translated into improved outcomes?

Bunn PA, Minna J, Augustyn A, Gazdar A, Ouadah Y, Krasnow MA, Berns A, Brambilla E, Rekhtman N, Massion PP, Niederst M, Peifer M, Yokota J, Govindan R, Poirier J, Byers LA, Wynes MW, McFadden D, MacPherson D, Hann CL, Farago AF, Dive C, Teicher BA, Peacock C, Johnson JE, Cobb MH, Wendel HG, Spigel D, Sage J, Yang P, Pietanza MC, Krug LM, Heymach J, Ujhazy P, Zhou C, Goto K, Dowlati A, Christensen CL, Park K, Einhorn LH, Edelman MJ, Giaccone G, Gerber DE, Salgia R, Owonikoko T, Malik S, Karachaliou N, Gandara DR, Slotman BJ, Blackhall F, Goss G, Thomas R, Rudin CM, Hirsch FR 2016 Jan J Thorac Oncol

Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Non-Muscle Myosin II.

Barry DM, Koo YS, Norden PR, Wylie LA, Xu K, Wichaidit C, Azizoglu DB, Zheng Y, Cobb MH, Davis G, Cleaver O 2016 Aug Circ. Res.

A Kinase Divided.

Karra AS, Taylor CA, Thorne CA, Cobb MH 2015 Aug Cancer Cell 2 28 145-7

Differential Regulation of ERK1/2 and mTORC1 Through T1R1/T1R3 in MIN6 Cells.

Wauson EM, Guerra ML, Dyachok J, McGlynn K, Giles J, Ross EM, Cobb MH 2015 Aug Mol. Endocrinol. 8 29 1114-1122

ASCL1 is a lineage oncogene providing therapeutic targets for high-grade neuroendocrine lung cancers.

Augustyn A, Borromeo M, Wang T, Fujimoto J, Shao C, Dospoy PD, Lee V, Tan C, Sullivan JP, Larsen JE, Girard L, Behrens C, Wistuba II, Xie Y, Cobb MH, Gazdar AF, Johnson JE, Minna JD 2014 Sep Proc. Natl. Acad. Sci. U.S.A.

G protein-coupled receptors and the regulation of autophagy.

Wauson EM, Dbouk HA, Ghosh AB, Cobb MH 2014 May Trends Endocrinol. Metab. 5 25 274-282

Ras transformation uncouples the kinesin-coordinated cellular nutrient response.

Zaganjor E, Weil LM, Gonzales JX, Minna JD, Cobb MH 2014 Jul Proc. Natl. Acad. Sci. U.S.A. 29 111 10568-73

ERK5 signaling gets XIAPed: a role for ubiquitin in the disassembly of a MAPK cascade.

Klein AM, Cobb MH 2014 Jul EMBO J.

NeuroD1 mediates nicotine-induced migration and invasion via regulation of the nicotinic acetylcholine receptor subunits in a subset of neural and neuroendocrine carcinomas.

Osborne JK, Guerra ML, Gonzales JX, McMillan EA, Minna JD, Cobb MH 2014 Apr Mol. Biol. Cell

Regulation of OSR1 and the sodium, potassium, two chloride cotransporter by convergent signals.

Sengupta S, Lorente-Rodríguez A, Earnest S, Stippec S, Guo X, Trudgian DC, Mirzaei H, Cobb MH 2013 Nov Proc. Natl. Acad. Sci. U.S.A. 47 110 18826-31

Ras regulates kinesin 13 family members to control cell migration pathways in transformed human bronchial epithelial cells.

Zaganjor E, Osborne JK, Weil LM, Diaz-Martinez LA, Gonzales JX, Singel SM, Larsen JE, Girard L, Minna JD, Cobb MH 2013 Nov Oncogene

Amino acid regulation of autophagy through the GPCR TAS1R1-TAS1R3.

Wauson EM, Zaganjor E, Cobb MH 2013 Mar Autophagy 3 9 418-9

Off-Target Effects of MEK Inhibitors.

Wauson EM, Guerra ML, Barylko B, Albanesi JP, Cobb MH 2013 Jul Biochemistry

Chromatin-tethered MAPKs.

Klein AM, Zaganjor E, Cobb MH 2013 Feb Curr. Opin. Cell Biol.

Minireview: nutrient sensing by g protein-coupled receptors.

Wauson EM, Lorente-Rodríguez A, Cobb MH 2013 Aug Mol. Endocrinol. 8 27 1188-97

NeuroD1 regulates survival and migration of neuroendocrine lung carcinomas via signaling molecules TrkB and NCAM.

Osborne JK, Larsen JE, Shields MD, Gonzales JX, Shames DS, Sato M, Kulkarni A, Wistuba II, Girard L, Minna JD, Cobb MH 2013 Apr Proc. Natl. Acad. Sci. U.S.A. 16 110 6524-9

NeuroD1 regulation of migration accompanies the differential sensitivity of neuroendocrine carcinomas to TrkB inhibition.

Osborne JK, Larsen JE, Gonzales JX, Shames DS, Sato M, Wistuba II, Girard L, Minna JD, Cobb MH 2013 Oncogenesis 2 e63

Ras regulates kinesin 13 family members to control cell invasion pathways in cancer.

E. Zaganjor, J.K. Osborne, L. Weil, L.A. Diaz-Martinez, J.X. Gonzales, S.M. Singel, J.E. Larsen, L. Girard, J.D. Minna, M.H. Cobb 2013 in press

Interactions with WNK (With No Lysine) Family Members Regulate Oxidative Stress Response 1 and Ion Co-transporter Activity.

Sengupta S, Tu SW, Wedin K, Earnest S, Stippec S, Luby-Phelps K, Cobb MH 2012 Nov J. Biol. Chem. 45 287 37868-79

Signal control through Raf: in sickness and in health.

Osborne JK, Zaganjor E, Cobb MH 2012 Jan Cell Res. 1 22 14-22

Interactions with with no lysine (K) (WNK) family members regulate oxidative stress response 1 (OSR1) and ion cotransporter activity

S. Sengupta, S.-W. Tu, K. Wedin, S. Earnest, S. Stippec, K. Luby-Phelps, M.H. Cobb 2012 J Biol Chem 37868-37879

WNK1 is required for mitosis and abscission.

Tu SW, Bugde A, Luby-Phelps K, Cobb MH 2011 Jan Proc. Natl. Acad. Sci. U.S.A. 4 108 1385-90

A small molecule differentiation inducer increases insulin production by pancreatic ? cells.

Dioum EM, Osborne JK, Goetsch S, Russell J, Schneider JW, Cobb MH 2011 Dec Proc. Natl. Acad. Sci. U.S.A. 51 108 20713-8

Regulation of CCAAT/enhancer-binding protein homologous protein (CHOP) expression by interleukin-1 beta in pancreatic beta cells.

Shao C, Lawrence MC, Cobb MH 2010 Jun J. Biol. Chem. 26 285 19710-9

Exposing contingency plans for kinase networks.

Klein AM, Dioum EM, Cobb MH 2010 Dec Cell 6 143 867-9

Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members.

Heise CJ, Xu BE, Deaton SL, Cha SK, Cheng CJ, Earnest S, Sengupta S, Juang YC, Stippec S, Xu Y, Zhao Y, Huang CL, Cobb MH 2010 Aug J. Biol. Chem. 33 285 25161-7

Regulation of CCAAT-enhancer binding protein homologous protein (CHOP) expression by interleukin 1 beta in pancreatic beta cells.

C. Shao, M.C. Lawrence and M.H. Cobb. 2010 285 19710-19719

Radial spoke protein 3 is a mammalian A kinase anchoring protein that binds ERK1/2

A. Jivan, S. Earnest, Y.C. Juang, and M.H. Cobb. 2009 284 29437-29445

Multiple chromatin-bound protein kinases assemble factors that regulate insulin gene transcription.

M.C. Lawrence, C. Shao, K. McGlynn, B. Naziruddin, M.F. Levy, and M.H. Cobb. 2009 106 22181-22186

Mutations in ERK2 binding sites affect nuclear entry.

Yazicioglu MN, Goad DL, Ranganathan A, Whitehurst AW, Goldsmith EJ, Cobb MH 2007 Sep J. Biol. Chem. 39 282 28759-67

Biological cross-talk between WNK1 and the transforming growth factor beta-Smad signaling pathway.

Lee BH, Chen W, Stippec S, Cobb MH 2007 Jun J. Biol. Chem. 25 282 17985-96

Mxi2 promotes stimulus-independent ERK nuclear translocation.

Casar B, Sanz-Moreno V, Yazicioglu MN, Rodríguez J, Berciano MT, Lafarga M, Cobb MH, Crespo P 2007 Feb EMBO J. 3 26 635-46

TAOs mediate activation of p38 in response to DNA damage.

M. Raman, S. Earnest, K. Zhang, Y. Zhao, and M.H. Cobb. 2007 26 2005-2014

WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells.

Anselmo AN, Earnest S, Chen W, Juang YC, Kim SC, Zhao Y, Cobb MH 2006 Jul Proc. Natl. Acad. Sci. U.S.A. 29 103 10883-8

Starvation activated MAPK through the muscarinic acetylcholine pathway in Caenorhabitis elegans pharynx

Y-j. You, J. Kim, M. Cobb, and L. Avery. 2006 3 237-245

WNK1 activates SGK1 to regulate the epithelial sodium channel.

Xu BE, Stippec S, Chu PY, Lazrak A, Li XJ, Lee BH, English JM, Ortega B, Huang CL, Cobb MH 2005 Jul Proc. Natl. Acad. Sci. U.S.A. 29 102 10315-20

WNK1 phosphorylates synaptotagmin 2 and regulates its ability to associate with membrane vesicles.

B-H. Lee, X. Min, B-e. Xu, S. Chen, H. Shu, E.J. Goldsmith, and M.H. Cobb. 2004 15 741-751

Regulation of insulin gene transcription by ERK1 and ERK2 in pancreatic beta cells.

Khoo S, Griffen SC, Xia Y, Baer RJ, German MS, Cobb MH 2003 Aug J. Biol. Chem. 35 278 32969-77

Activation mechanism of the MAP kinase ERK2 by dual phosphorylation.

B.J. Canagarajah, A. Khokhlatchev, M.H. Cobb, and E.J. Goldsmith. 1997 90 859-869

Tubulointerstitial injury induced in rats by a monoclonal antibody that inhibits function of a membrane inhibitor of complement.

Nomura A, Nishikawa K, Yuzawa Y, Okada H, Okada N, Morgan BP, Piddlesden SJ, Nadai M, Hasegawa T, Matsuo S, 1995 Nov J Clin Invest 5 96 2348-56

ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.

T.G. Boulton, S.H. Nye, D.J. Robbins, N.Y. Ip, E. Radziejewska, S.D. Morgenbessor, R.A. DePinho, N. Panayotatos, M.H. Cobb and G.D. Yancopoulos. 1991 65 663-675