Publications

Original Research 

BN Kakde, E Capota, JJ Kohler*, UK Tambar*. Synthesis of cell-permeable N-acetylhexosamine-1-phosphates, J. Org. Chem. (2021) doi.org/10.1021/acs.joc.1c01781

N Pedowitz, E Jackson, J Overhulse, C McKenna, JJ Kohler, MR Pratt. Anomeric fatty-acid functionalization prevents non-enzymatic S-glycosylation by monosaccharide metabolic chemical reporters. ACS Chem. Biol. (2021) 16: 1924–1929

H Wu  A Shajahan, J-Y Yang, E Capota, AM Wands, CM Arthur, SR Stowell, KW Moremen, P Azadi, JJ Kohler. A photocrosslinking GlcNAc analog enables covalent capture of N-linked glycoprotein binding partners. Cell Chem. Biol. (2021) doi:10.1016/j.chembiol.2021.07.007

E Capota, H Wu, JJ Kohler. Photocrosslinking O-GlcNAcylated proteins to neighboring biomolecules. Current Protocols (2021) doi:10.1002/cpz1.201

JJ Kohler. What sugar does to your pores Cell Biol. (2021) 220: e202105163

Shajahan A, Supekar NT, Wu H, et al. Mass spectrometric method for the unambiguous profiling of cellular dynamic glycosylation [published online ahead of print, 2020 Aug 18]. ACS Chem Biol. 2020;10.1021/acschembio.0c00453. doi:10.1021/acschembio.0c00453

B Schumann, SA Malaker, SP Wisnovsky, MF Debets, AJ Agbay, D Fernandez, LJS Wagner, L Lin, J Choi, DM Fox, JM Peh, MA Gray, K Pedram, JJ Kohler, M Mrksich, CR Bertozzi.  Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells.  Molecular Cell  (2020) 78: 824-834

Broussard A, Florwick A, Desbiens C, Nischan N, Robertson C, Guan Z, Kohler JJ, Wells L, Boyce M. The human UDP-galactose 4’-epimerase (GALE) is required for cell-surface glycome structure and function. J. Biol. Chem. (2019). doi:10.1074/jbc.RA119.009271 

Wu H  and Kohler J. Photocrosslinking probes for capture of carbohydrate interactions. Current Opinion in Chemical Biology. (2019). 53: 173-182

Ghorashi AC and Kohler JJ. Not all quiet on the sugar front: Glycan combatants in host-pathogen interactions. Biochemistry. (2019).

Sethi A, Wands AM, Mettlen M, Krishnamurthy S, Wu H, Kohler JJ. Cell type and receptor identity regulate cholera toxin subunit B (CTB) internalization. Interface Focus. (2019). 9: 20180076

CA Toleman, MA Schumacher, S-H Yu, W Zeng, NJ Cox, TJ Smith, EJ Soderblom, AM Wands, JJ Kohler, M Boyce.  Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins. Proc. Natl. Acad. Sci. U. S. A. (2018). doi: 10.1073/pnas.1722437115

J Cervin, AM Wands, A Casselbrant, H Wu, S Krishnamurthy, A Cvjetkovic, J Estelius, B Dedic, A Sethi, K-L, Wallom, R Riise, M Bäckström, V Wallenius, FM Platt, M Lebens, S Teneberg, L Fändriks, JJ Kohler, U Yrlid. GM1 ganglioside-independent intoxication by cholera toxin. PLoS Pathogens (2018). 14:e1006862 * featured on PLoS Cholera Channel

AM Wands, J Cervin, H Huang, Y Zhang, G Youn, CA Brautigam, M Matson Dzebo, P Björklund, V Wallenius, DK Bright, CS Bennett, P Wittung-Stafshede, NS Sampson, U Yrlid, JJ Kohler. Fucosylated molecules competitively interfere with cholera toxin binding to host cells. ACS Infect. Dis. (2018). 4:758-770  * highlighted in ScienceDaily; www.sciencedaily.com/releases/2018/03/180309100653.htm

K Tanigaki, A Sacharidou, J Peng, KL Chambliss, IS Yuhanna, D Ghosh, M Ahmed, AJ Szalai, W Vongpatanasin, RF Mattrey, Q Chen, P Azadi, I Lingvay, M Botto, WL Holland, JJ Kohler, SR Sirsi, K Hoyt, PW Shaul, C Mineo.  Hyposialylated IgG activates endothelial IgG receptor FcγRIIB to promote obesity-induced insulin resistance. J. Clin. Invest. (2018) 128:309-322

S-K Park, X Zhou, K Pendleton, OV Hunter, JJ Kohler, KA O’Donnell, and NK Conrad.  A conserved splicing silencer dynamically regulates O-GlcNAc transferase intron retention and O-GlcNAc homeostasis. Cell Rep. (2017) 20:1088-1099

Lissette M. Andres, Ian W. Blong, Angela C. Evans, Neil G. Rumachik, Teppei Yamaguchi, Nam D. Pham, Pamela Thompson, Jennifer J. Kohler, and Carolyn R Bertozzi. Chemical modulation of protein O-GlcNAcylation via OGT inhibition promotes human neural cell differentiation. ACS Chemical Biology. (2017). 12(8):2030-2039. doi: 10.1021/acschembio.7b00232.  

Nam D. Pham, Poh-Choo Pang, Soumya Krishnamurthy, Amberlyn M. Wands, Paola Grassi, Anne Dell, Stuart M. Haslam, and Jennifer J. Kohler. Effects of Altered Sialic Acid Biosynthesis on N-linked Glycan Branching and Cell Surface Interactions. Journal of Biological Chemistry 2017 Apr 19. pii: jbc.M116.764597

Jon D. Wright, Sung-Wan An, Jian Xie, Joonho Yoon, Nicole Nischan, Jennifer J. Kohler, Noelynn Oliver, Carmay Lim, and Chou-Long Huang. Modeled Structural Basis for the Recognition of α2-3-Sialyllactose by Soluble Klotho. FASEB Journal. 2017 Apr 25. pii: fj.201700043R.

George Dalton, Sung-Wan An, Saif I. Al-Juboori, Nicole Nischan, Joonho Yoon, Evgenia Dobrinskikh, Donald W. Hilgemann, Jian Xie, Kate Luby-Phelps, Jennifer J. Kohler, Lutz Birnbaumer, and Chou-Long Huang. Soluble klotho binds monosialoganglioside to regulate membrane microdomains and growth factor signaling. Proceedings of the National Academy of Sciences USA, (2017) 2017 Jan 24;114(4):752-757

Christopher Leija, Filipa Rijo-Ferreira, Lisa N. Kinch, Nick V. Grishin, Nicole Nischan, Jennifer J. Kohler, Zeping Hu, and Margaret A. Phillips. Pyrimidine Salvage Enzymes Are Essential for De Novo Biosynthesis of Deoxypyrimidine Nucleotides in.Trypanosoma brucei PLoS Pathogens 2016 Nov 7;12(11):e1006010

Janet E. McCombs, Jason P. Diaz, Kevin J. Luebke, and Jennifer J. Kohler. Glycan specificity of neuraminidases determined in microarray format. Carbohydrate Research. (2016) Apr 8;428:31-40.

Janet E. McCombs and Jennifer J. Kohler. Pneumococcal neuraminidase substrates identified through comparative proteomics enabled by chemoselective labeling. Bioconjugate Chemistry, (2016) 27 (4), pp 1013–1022

Janet E. McCombs, Chunxia Zou, Randy B. Parker, Christopher W. Cairo, and Jennifer J. Kohler. Enhanced crosslinking of diazirine-modified sialylated glycoproteins enabled through profiling of sialidase specificities. ACS Chemical Biology. (2016) Jan 15;11(1):185-92.

Amberlyn M Wands,* Akiko Fujita,* Janet E McCombs, Jakob Cervin, Benjamin Dedic, Andrea C Rodriguez, Nicole Nischan, Michelle R Bond, Marcel Mettlen, David C Trudgian, Andrew Lemoff, Marianne Quiding-Järbrink, Bengt Gustavsson, Catharina Steentoft, Henrik Clausen, Hamid Mirzaei, Susann Teneberg, Ulf Yrlid, and Jennifer J Kohler. Fucosylation and protein glycosylation create functional receptors for cholera toxin. eLIFE. (2015) Doi: 10.7554/eLife.09545 (*=Equal contribution)

Andrea C. Rodriguez, Seok-Ho Yu, Bin Li, Hicham Zegzouti, and Jennifer J. Kohler. Enhanced Transfer of a Photocrosslinking GlcNAc Analog by an O-GlcNAc Transferase Mutant with Converted Substrate Specificity. Journal of Biological Chemistry. (2015) 290: 22638-22648.

Nam D. Pham, Charles S. Fermaintt,* Andrea C. Rodriguez,* Janet E. McCombs, Nicole Nischan, and Jennifer J. Kohler. Cellular Metabolism of Unnatural Sialic Acid Precursors. Glycoconj. J. (2015) 32: 515-529 (*=Equal contribution)

Andrea C. Rodriguez and Jennifer J. Kohler. Recognition of diazirine- modified O-GlcNAc by human O-GlcNAcase. Med. Chem. Comm., (2014) 5(8): 1227- 1234. 

Randy B. Parker, Janet E. McCombs, and Jennifer J. Kohler. Sialidase specificity determined by chemoselective modification of complex sialylated glycans. ACS Chemical Biology (2012) 7: 1509-1514.

Seok-Ho Yu, Michael Boyce, Amberlyn M. Wands, Michelle R. Bond, Carolyn R. Bertozzi, and Jennifer J. Kohler. Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners, Proceedings of the National Academy of Sciences USA, (2012) 109: 4834-4839.

Michelle R. Bond, Haochi Zhang, Jaekuk Kim, Seok-Ho Yu, Fan Yang, Steven M. Patrie, and Jennifer J. Kohler.Metabolism of diazirine-modified N-acetylmannosamine analogs to photocrosslinking sialoside, Bioconjugate Chemistry, (2011) 22: 1811-1823.

Chad M. Whitman, Fan Yang, and Jennifer J. Kohler. Modified GM3 gangliosides produced by metabolic oligosaccharide engineering, Bioorganic & Medicinal Chemistry, (2011) 21: 5006–5010.

Michael Boyce, Isaac S. Carrico, Anjali S. Ganguli, Seok-Ho Yu, Matthew J. Hangauer, Sarah C. Hubbard, Jennifer J. Kohler, and Carolyn R. Bertozzi. Metabolic crosstalk allows labeling of human O-linked β-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway, Proc. Natl. Acad. Sci. USA, (2011) 108: 3141-3146.

Danielle H. Dube,* Bin Li,* Ethan J. Greenblatt, Sadeieh Nimer, Amanda K. Raymond, and Jennifer J. Kohler. A two-hybrid assay to study protein interactions within the secretory pathway,PLoS ONE (2010) 5: e15648. (*=Equal contribution)

Michelle R. Bond, Chad M. Whitman, and Jennifer J. Kohler. Metabolically incorporated photocrosslinking sialic acid covalently captures a ganglioside-protein complex, Molecular Biosystems (2010) 6: 1796-1799.

Marguerite M. Desko, David A. Gross, and Jennifer J. Kohler. Effects of N-glycosylation on the activity and localization of GlcNAc-6-sulfotransferase 1, Glycobiology, (2009) 19: 1068-1077.

Michelle R. Bond, Haochi Zhang, Peter D Vu & Jennifer J. Kohler. Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars, Nature Protocols (2009) 4: 1044-1063.

Peter L. Lee, Jennifer J. Kohler, and Suzanne R. Pfeffer. Association of β-1,3-N-acetylglucosaminyltransferase 1, iGnT, and β-1,4-galactosyltransferase 1, GalT1, trans-Golgi glycosyltransferases involved in poly-N-acetyllactosamine synthesis, Glycobiology (2009) 12: 655-664.

Yoshihito Tanaka, and Jennifer J. Kohler. Photoactivatable crosslinking sugars for capturing glycoprotein interactions, J. Amer. Chem. Soc. (2008) 130: 3278-3279.

Reviews, Book Chapters & Commentary

Jennifer J. Kohler. "Carb cutting works better with a partner". Nature Structural & Molecular Biology 24, 433–435 (2017)

Nicole Nischan, and Jennifer J. Kohler. "Advances in Cell Surface Glycoengineering Reveal Biological Function". Glycobiology (2016) 2016 Apr 10. pii: cww045

Akiko Fujita A, and Jennifer J. Kohler. "Metabolism of Natural and Unnatural Sialic Acid." Glycoscience: Biology and Medicine. In press. 

Janet E. McCombs, and Jennifer J. Kohler. "Chemoselective reactions for glycan labeling," Chemoselective and Bioorthogonal Ligation Chemistries: Concepts and Applications. Edited by R Algar, P Dawson, I Medintz. Wiley-VCH: Weinheim, Germany. In press.

Bin Li, and Jennifer J. Kohler. Glycosylation of the Nuclear Pore. Traffic, (2014) 15: 347- 31. 

Jennifer J. Kohler JJ, and Steven M Patrie, eds. Mass Spectrometry of Glycoproteins: Methods and Protocols, Methods in Molecular Biology Series (2012) Humana Press. Available November 2012.

Nam D. Pham, Randy B. Parker, and Kohler JJ. Photocrosslinking approaches to interactome mapping. Curr. Op. Chem. Biol., (2012) 17: 1- 12.

Seok-Ho Yu, Amberlyn M. Wands, and Jennifer J. Kohler. Photoaffinity probes for studying carbohydrate biology. Journal of Carbohydrate Chemistry (2012) 31: 325-352.

Jennifer J. Kohler. Post-translational modifications: A shift for the O-GlcNAc paradigm (News & Views)., Nat. Chem. Biol., (2010) 5: 634-635.

Seok-Ho Yu, Michelle R. Bond, Chad M. Whitman, and Jennifer J. Kohler. Metabolic labeling of glycoconjugates with photocrosslinking sugars, Methods in Enzymology (2010) 478: 541-562.

Chad M. Whitman, Michelle R. Bond, and Jennifer J. Kohler. Chemical glycobiology (Book Chapter), Comprehensive Natural Products Chemistry II (2010).

Randy B. Parker, and Jennifer J. Kohler. Regulation of intracellular signaling by extracellular glycan remodeling, ACS Chemical Biology (2010) 5: 35-46.

Jennifer J. Kohler. Aniline: a catalyst for sialic acid detection, ChemBioChem, (2009) 10: 2147-2150.

Marguerite M Desko, and Jennifer J. Kohler. Glycosylation of proteins in the Golgi apparatus(Book Chapter), Wiley Encyclopedia of Chemical Biology (2008).

Yoshihito Tanaka, Michelle R. Bond, and Jennifer J. Kohler. Photocrosslinkers illuminate interactions in living cells (Review Article), Molecular Biosystems (2008) 4: 473-480.

Jason W. Chin, & Jennifer J. Kohler. Current and future prospects in biopolymer chemistry (Editorial Overview), Curr. Op. Chem. Biol. (2007) 11: 626-627.

Jennifer J. Kohler. Chemical biology meets networks (Book Review), Nat. Chem. Biol. (2007) 3: 528-529.

Michelle R. Bond, and Jennifer J. Kohler. Chemical methods for glycoprotein discovery(Review Article), Curr. Op. Chem. Biol. (2007) 11: 52-58.

Danielle H. Dube, Christopher L. de Graffenried, Jennifer J. Kohler. Regulating cell surface glycosylation with a small molecule switch, Methods in Enzymology (2006) 415: 213-229.

Jennifer J. Kohler. A century at the chemistry-biology interface, Nat. Chem. Biol. (2006) 2: 288-292.