Publications

Featured Publications

Fic Null Pancreatic Fibrosis and UPR Model

August 1, 2022

Fic-mediated AMPylation tempers the unfolded protein response during physiological stress.

Casey AK, Gray HF, Chimalapati S, Hernandez G, Moehlman AT, Stewart N, Fields HA, Gulen B, Servage KA, Stefanius K, Blevins A, Evers BM, Krämer H, Orth K.

Description

We generated a conditional null allele of the AMPylating enzyme filamentation induced by cyclic-AMP (Fic) in mice and characterized the effect of Fic loss on the exocrine pancreas. We reveal Fic is required in differentiated tissue for the regulation of the unfolded protein response (UPR) during both physiological and pharmacological stresses. We also propose that cells with high regenerative capacity may not require this level of regulation, as new cells will bypass the need for long-term survival. 

Co-component signal transduction system

June 14, 2022

Co-component signal transduction systems: Fast-evolving virulence regulation cassettes discovered in enteric bacteria

Lisa N. Kinch, Qian Cong, Jananee Jaishankar, and Kim Orth

Description

We identified co-component signal transduction systems in enteric bacteria that are distantly related to the periplasmic bile acid receptor VtrA/VtrC from Vibrio parahaemolyticus. The newly identified co-component systems, which include the ToxR/ToxS master regulator of virulence, were identified using domain and operon organization combined with newly developed machine learning methods for structure prediction. The lipocalin-like fold adopted by all VtrC-like components suggests a regulatory role for sensing lipids in the virulence of enteric bacteria.

VopQ effector bound to Vo ATPase

August 18, 2020

Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress.

Suneeta Chimalapati, Marcela de Souza Santos, Alexander E Lafrance, Ann Ray, Wan-Ru Lee, Giomar Rivera-Cancel, Gonçalo Vale, Krzysztof Pawlowski, Matthew A Mitsche, Jeffrey G McDonald, Jen Liou, Kim Orth

Description

Using a combination of molecular tools, we found that VPA0226, a constitutively secreted lipase, is required for escape of V. parahaemolyticus from the host cells. This lipase must be delivered into the host cytoplasm where it preferentially uses fatty acids associated with innate immune response to esterify cholesterol. The activity weakens the plasma membrane and allows egress of the bacteria.

VopQ effector bound to Vo ATPase

May 18, 2020

A distinct inhibitory mechanism of the V-ATPase by Vibrio VopQ revealed by cryo-EM.

Wei Peng, Amanda K Casey, Jessie Fernandez, Emily M Carpinone, Kelly A Servage, Zhe Chen, Yang Li, Diana R Tomchick, Vincent J Starai, Kim Orth

Description

The Vibrio parahaemolyticus T3SS effector VopQ targets host-cell V-ATPase, resulting in blockage of autophagic flux and neutralization of acidic compartments. Here, we report the cryo-EM structure of VopQ bound to the Vo subcomplex of the V-ATPase. 

Fic null fly eyes SEM images

December 22, 2017

Fic-mediated deAMPylation is not dependent on homodimerization and rescues toxic AMPylation in flies.

Amanda K Casey, Andrew T Moehlman, Junmei Zhang, Kelly A Servage, Helmut Krämer, Kim Orth

Description

We show that excessive AMPylation by a constitutively active FicE247G mutant is lethal in Drosophila. This lethality is cell-autonomous, as directed expression of the mutant FicE247G to the fly eye does not kill the fly but rather results in a rough and reduced eye. Lethality and eye phenotypes are rescued by the deAMPylation activity of wild-type Fic.

July 5, 2016

Bile salt receptor complex activates a pathogenic type III secretion system

Peng Li, Giomar Rivera-Cancel, Lisa N Kinch, Dor Salomon, Diana R Tomchick, Nick V Grishin, Kim Orth

Description

We report the crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer. The VtrC fold adopts a β-barrel with a hydrophobic inner chamber. We present biophysical and mutational analysis to demonstrate the hydrophobic chamber binds bile salts and activates the T3SS2 virulence network. 

VtrA/VtrC bile receptor structure
VopS Fic domain AMPylation of host GTPases causes cell rounding

January 9, 2009

AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling

Melanie L Yarbrough, Yan Li, Lisa N Kinch, Nick V Grishin, Haydn L Ball, Kim Orth

Description

We found the Vibrio parahaemolyticus type III effector VopS Fic domain covalently modifies a conserved threonine residue on Rho, Rac, and Cdc42 GTPases with adenosine 5′-monophosphate (AMP). The resulting AMPylation prevented the interaction of Rho GTPases with downstream effectors to inhibit actin assembly in the infected cell. This discovery expanded the repertoire of posttranslational modifications for molecular signaling and established a paradigm for catalysis by Fic domains.

All Publications

Publications by Year

2022

  • Lafrance AE, Chimalapati S, Garcia Rodriguez N, Kinch LN, Kaval KG, Orth K. Enzymatic Specificity of Conserved Rho GTPase Deamidases Promotes Invasion of Vibrio parahaemolyticus at the Expense of Infection. mBio. 2022 Aug 30;13(4):e0162922.
  • Casey AK, Gray HF, Chimalapati S, Hernandez G, Moehlman AT, Stewart N, Fields HA, Gulen B, Servage KA, Stefanius K, Blevins A, Evers BM, Krämer H, Orth K. Fic-mediated AMPylation tempers the unfolded protein response during physiological stress. Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2208317119. doi: 10.1073/pnas.2208317119. 
  • Kinch LN, Cong Q, Jaishankar J, Orth K. Co-component signal transduction systems: Fast-evolving virulence regulation cassettes discovered in enteric bacteria. Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2203176119. doi: 10.1073/pnas.2203176119.

2021

  • Ebrahimzadeh T, Kuprasertkul A, Neugent ML, Lutz KC, Fuentes JL, Gadhvi J, Khan F, Zhang C, Sharon BM, Orth K, Li Q, Zimmern PE, De Nisco NJ. Urinary prostaglandin E2 as a biomarker for recurrent UTI in postmenopausal women. Life Sci Alliance. 2021 May 6;4(7):e202000948. doi: 10.26508/lsa.202000948.
  • De Nisco, N. J., Casey, A. K., Kanchwala, M., Lafrance, A. E., Coskun, F. S., Kinch, L. N., . . . & Orth, K. (2021). Manipulation of ire1-dependent mapk signaling by a vibrio agonist-antagonist effector pair. MSystems, 6(1). doi:10.1128/msystems.00872-20
  • Fernandez, J., Lopez, V., Kinch, L., Pfeifer, M. A., Gray, H., Garcia, N., . . . & Orth, K. (2021). Role of two metacaspases in development and pathogenicity of the Rice Blast fungus Magnaporthe oryzae. MBio, 12(1). doi:10.1128/mbio.03471-20
  • Stefanius, K., Servage, K., & Orth, K. (2021). Exosomes in cancer development. Current Opinion in Genetics & Development, 66, 83-92. doi:10.1016/j.gde.2020.12.018

2020

  • Chimalapati, S., de Souza Santos, M.*, Lafrance, A., Ray, A., Lee, W-R., Rivera-Cancel, G., Vale, G., Pawłowski, K., Mitsche, M., McDonald, J.G. , Liou, J. & Orth, K. Vibrio deploys a Type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate T3SS2-mediated cell egress. eLife, 9:e58057.
  • Peng, W., Fernandez, J., Casey, A. K., Kinch, L. N., Tomchick, D. R., & Orth, K. (2020). Structures of an intact yeast v-atpase alone and in complex with bacterial effector vopq. doi:10.1101/2020.07.16.207225
  • Peng, W., Casey, A. K., Fernandez, J., Carpinone, E. M., Servage, K. A., Chen, Z., Li, Y., Tomchick, D. R., Starai, V. J., & Orth, K. (2020). A distinct inhibitory mechanism of the V-ATPase by Vibrio VopQ revealed by cryo-EM. Nature structural & molecular biology, 10.1038/s41594-020-0429-1. Advance online publication.
  • Servage, K. A., Stefanius, K., Gray, H. F., & Orth, K. (2020). Proteomic Profiling of Small Extracellular Vesicles Secreted by Human Pancreatic Cancer Cells Implicated in Cellular Transformation. Scientific reports10(1), 7713.

2019

  • Yang, H.*, de Souza Santos, M.*, Lee, J., Law, H.T., Chimalapati, S., Verdu, E.F., Orth, K. #, and Vallance, B.A.# A novel mouse model of enteric Vibrio parahaemolyticus infection reveals the T3SS2effector VopC plays a key role in tissue invasion and gastroenteritis. (2019) *contributed equally, #co-corresponding. mBio 17;10(6). pii: e02608-19. doi: 10.1128/mBio.02608-19.
  • Stefanius, K., Servage, K., de Souza Santos, M., Chimalapati, S., Gray, H., Kim, M., Brekken, R. & Orth, K. Pancreatic Cancer Cell Exosomes Initiate Cellular Transformation and Tumor formation. eLife, (2018) May 28;8. pii: e40226. doi: 10.7554/eLife.40226.
  • Peng, W., de Souza Santos, M., Tomchick, D. & Orth, K. Assembly mechanism of the E. coli hemolysin ClyA pore complexes. PLoS ONE (2019) May 2;14(5):e0213423. doi: 10.1371/journal.pone.0213423.
  • De Nisco, N., Neugent, M., Mull, J., Chen, L., Kuprasertkul, A., de Souza Santos, M., Palmer, K., Zimmern, P. & Orth, K. Direct detection of tissue-resident bacteria and chronic inflammation in the bladder wall of postmenopausal women with recurrent urinary tract infection. Journal of Molecular Biology (2019) Apr 17. pii: S0022-2836(19)30202-5.

2018

  • Sreelatha, A., Yee, S.S., Lopez, V.A., Park, B.C., Kinch, L., Pilch, S., Servage, K.A., Zhang, J., Jiou, J., Karasiewicz, M., M?obocka, M., Grishin, N., Orth, K, Kucharczyk, R., Paw?owski, K., Tomchick, D. R., and Tagliabracci. V.S. Protein AMPylation by an evolutionarily conserved pseudokinase. Cell. (2018) 175(3):809-821
  • Moehlman, A.T., Casey, A.K., Servage, K., Orth, K* & Krämer, H.* Regulation of the Unfolded Protein Response by BiP AMPylation protects photoreceptors from light-dependent degeneration. eLife. (2018) Jul 17:7. Pii:e38752. doi:10.7554/eLife.38752. PMID: 30015618.
  • Orth, K. My winding trail while fulfilling my love for science and family. J Biol Chem.. (2018) pii: jbc.AW118.003225. doi: 10.1074/jbc.AW118.003225
  • Chimalapati,S., de Souza Santos, M., Servage, K., De Nisco, N.J., Dalia, A.B. & Orth, K. Natural transformation of Vibrio parahaemolyticus: A rapid method to create genetic deletions. J. Bac. 2018 Jul 10;200(15). pii: e00032-18. doi: 10.1128/JB.00032-18
  • Fernandez, J. & Orth, K. Rise of a Cereal Killer: The Biology of Magnaporthe oryzae Biotrophic Growth. Trends in Microbiology (2018)
  • De Nisco, N.J., Rivera-Cancel, G. & Orth, K. The biochemistry of sensing: How enteric pathogens regulate type III secretion in response to environmental and host cues. mBio. (2018) vol. 9 no. 1 e02122-17

2017

  • Ray, A., Schwartz, N., de Souza Santos, M., Zhang, J. Orth, K & Salomon, D. Type VI secretion system MIX-effectors are targeting at both Bacterial and eukaryotic neighbors. EMBO Report. (2017) e201744226
  • Casey, A.K. & Orth, K. Enzymes involved in AMPylation and deAMPylation. Chemical Reviews. (2017) Aug 18. doi: 10.1021/acs.chemrev.7b00145.
  • Cancel-Rivera, G. & Orth, K. Biochemical basis for activation of virulence genes by bile salts in Vibrio parahaemolyticusGut Microbes.. (2017) Jan 27:0. doi: 10.1080/19490976.2017.1287655
  • Casey, A.K., Moelman, A., Zhang, J., Servage, K., Kramer, H. & Orth, K. Fic-mediated deAMPylation of BiP is not dependent on homo-dimerization and rescues toxic AMPylation in flies. J Biol Chem. (2017) 292 (51) pg 21193-21204)
  • de Souza Santos, M., Salomon, D. & Orth, K. T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus. PLoS Pathog (2017) 13(6): e1006438. doi: 10.1371/journal.ppat.100643
  • Li,P., Salomon, D., Kinch, L.N., Ray, A., Dalia, A.B., Cong, Q., Ninan, L., Camilli, A., Grishin, N.V. & Orth, K. Acute Hepatopancreatic Necrosis Disease (AHPND) causing Vibrio parahaemolyticus contains a virulent Type VI Secretion System (T6SS) with anti-bacterial activities. Appl Environ Microbiol. (2017) Apr 21. pii: AEM.00737-17. doi: 10.1128/AEM.00737-17
  • De Nisco, N.J., Kanchwala, M., Li, P., Fernandez, J., Xing, C. & Orth, K. Cytotoxic Vibrio T3SS1 Rewires Host Gene Expression to Subvert Cell Death Signaling and Activate Cell Survival Networks. Sci Signal (2017) May 16;10(479). pii: eaal4501. doi: 10.1126/scisignal.aal4501
  • De Nisco, N.J., Orth, K. & VanHook, A.M., Signaling Science Podcast: Vibrio Rewires host cells. Sci Signal. 2017 May 16;10(479). pii: eaan5621. doi: 10.1126/scisignal.aan5621

2016

  • Huebinger, R.M., Stones, D.H., de Souza Santos, M., Carlson, D.L., Song, J., Vaz, D.P., Keen, E., Wolf, S.E., Orth, K. & Krachler, A. M. Targeting bacterial adherence inhibits multidrug-resistant Pseudomonas aeruginosa infection following burn injury. (2016) Scientific Reports Dec 20;6:39341. doi: 10.1038/srep39341
  • Li, P., Rivera-Cancel, G., Kinch, L.N., Salomon, D., Tomchick, D.R., Grishin, N.V. & Orth, K. Bile Salt Receptor Complex Activates a Pathogenic Type III Secretion System (2016) eLife 5:e15718.
  • Ray, A., Kinch, L.N., , de Souza Santos, M., Grishin, N.V., Orth, K. & Salomon, D. Proteomics analysis reveals previously uncharacterized virulence factors in Vibrio proteolyticus (2016) mBio 7(4):e01077-16. doi:10.1128/mBio.01077-16.

2015

  • Salomon, D., Klimko, J.A., Trudgian, D.C., Kinch, L.N., Grishin, N.V., Mirzaei, H & Orth, K. Type VI secretion system toxins horizontally shared between marine bacteria (2015) PLoS Pathogen Aug 25;11(8):e1005128. PMID 26305100.
  • Sreelatha, A., Bennett, T.L., Carpinone, E.M., O’Brien, K.M., Jordan, K.D., Burdette, D.L., Orth, K, & Starai, V.J. The Vibrio effector protein, VopQ, inhibits fusion of V-ATPase containing membranes. (2015) PNAS 112(1):100-5 PMID:25453092.

2014

  • Ham H., Woolery A.R., Tracy C., Stenesen D., Krämer H. & Orth, K. Unfolded protein response-regulated dFic reversibly AMPylates BiP during ER homeostasis. (2014) JBC 289(52):36059-69 PMID: 25395623.
  • Calder, T., Santos, M.S., Attah, V., Klimko, J., Frernandez, J., Salomon, D., Krachler, A.M., & Orth, K. Structural and regulatory mutations in Vibrio parahaemolyticus type III secretion systems display variable effects on virulence. (2014) FEMS 361(2):107-14. PMID 25288215.
  • Woolery, A.R., Yu, X., LaBaer, J.& Orth, K. AMPylation of Rho GTPases subverts multiple host signaling processes. (2014) JBC 289(47):32977-88. PMID 25301945.
  • de Souza Santos, M.S.. & Orth, K. Intracellular Vibrio parahaemolyticus Escapes the Vacuole and Establishes a Replicative Niche in the Cytosol of Epithelial Cells. (2014) mBio 9;5(5):e01506-14.
  • Yu, X., Woolery, A.R., Luong, P., Hao, Y.H., Grammel, M., Westcott, N., Park, J., Wang, J., Bian, X., Demirkan, G., Hang, H.C., Orth, K. & LaBaer, J. Click chemistry-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays. (2014) Mol & Cell Proteomics 13(11):3164-76. PMID 25073739.
  • Calder, T., Kinch, L.N., Fernandez, J., Salomon, D., Grishin, N.V., & Orth, K. Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxin PLoS ONE 6;9(8):e104387 PMID 25099122.
  • Salomon, D., Klimko, & Orth, K. H-NS regulates the Vibrio parahaemolyticus type VI secretion system 1. (2014) Microbiology 160(Pt9):1867-73. PMID:24987102.
  • Salomon, D., Kinch, L.N., Trudgian, D.C., Guo, X., Klimko, J.A., Grishin, N.V., Mirzaei, H  & Orth, K. Marker for type VI secretion system effectors. PNAS USA (2014) 111(25):9271-6 PMID 24927539.
  • Lewallen,D.M., Sreelatha, A., Dharmarajan, V., Madoux, F., Chase,, F. ,Griffin, P.R., Orth, K., Hoddler, P. & Thompson, P.R. Inhibiting AMPylation: a novel screen to identify the first small molecule inhibitors of protein AMPylation. ACS Chem Biol (2014) 9(2): 433-432 PMC3944102.

2013

  • Sreelatha, A., Orth, K., & Starai, V.J. The pore-forming bacterial effector, VopQ, halts autophagic turnover. Autophagy (2013) 9(12): 2169-70. PMID:24145145.
  • Salomon D., Guo, Y., Kinch, L.N., Grishin, N.V., Mirzaei, H. & Orth, K. Effectors of animal and plant pathogens use a common domain to bind host phosphoinositides. Nature Communications (2013) 4:2973. Doi:10.1038/n,comms3973.
  • Hawley, C.A., Watson, C., Orth, K. & Krachler, A-M. High-affinity binding of bacterial fibronectin-binding proteins to host cells inhibits critical steps in wound healing. PloS ONE (2013) 8.11:e81216.
  • Sreelatha, A., Bennett,T. L., Zheng, H., Jiang, Q.X., Orth, K.*, & Starai, V.J.* Vibrio VopQ forms a gated outward rectifying channel that disrupts host ion homeostasis. PNAS USA (2013) 110(28):11559-64.
  • Altura, M.A., Heath-Heckman, E.A., Gillette, A. Kremer, A-M, Krachler, A.M. Brennean, c., Ruby, E.G., Orth, K., McFall-Ngai, M.J. (2013) The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells. Environ Microbiol. (2013) Jun 11. doi: 10.1111/1462-2920.12179 (Epub ahead of print).
  • Salomon, D. & Orth, K. What Pathogens Have Taught Us About Posttranslational Modifications (2013) Cell Host Pathogens 14:3 269-279.
  • Krachler, A-M. & Orth, K. Made to stick: Anti-adhesion therapy for bacterial infections as an alternative to conventional antimicrobials. (2013) Microbe 8:286-290.
  • Krachler, A-M. & Orth, K. Targeting the bacteria-host interface: strategies in anti-adhesion therapy (2013) Virulence 4(4): 284-94.
  • Salomon, D. & Orth, K. Lost after translation: post-translational modifications by bacterial type III effectors. (2013) Current Opinions in Microbiology 6(2):213-20.
  • Zhang, L.L & Orth, K. Virulence Determinants for Vibrio parahaemolyticus Infection. Current Opinions in Microbiology (2013) 16(1):70-7.
  • Salomon, D., Gonzalez, H., Updegraff, B.L. & Orth, K. Vibrio parahaemolyticus Type VI Secretion System 1 is Activated in Marine Conditions to Target Bacteria, and is Differentially Regulated from System 2. PLoS ONE (2013) 8:4 e61086.

2012

  • Ham, H. & Orth, K. The Role of Type III Secretion System 2 in Vibrio parahaemolyticus Pathogenicity. J Microbiol. (2012) Oct;50(5):719-25. PMID:23124738[PubMed - in process]
  • Krachler AM & Orth, K. In vitro characterization of Multivalent Adhesion Molecule 7-based inhibition of multidrug-resistant bacteria isolated from wounded military personnel. Virulence (2012) (In Press)
  • Alto, N.M. & Orth, K. Subversion of cell signaling by pathogens. In Signal Transduction Textbook. Cold Spring Harbor Press (2012).
  • Zhang, L.L, Krachler, A-M, Broberg, CA, Li, Y, Mirzaei, H, Gilpin, CJ & Orth, K. Type III effector VopC mediates invasion for Vibrio species. Cell Reports (2012) 1(5): 453-460.
  • Rahman, M., Ham, H., Liu, X., Sugiura, Y., Orth, K. and Krämer, H. Visual neurotransmission in Drosophila requires expression of dFic in glial capitate projections. Nature Neuroscience (2012) Apr 29. doi: 10.1038/nn.3102. [Epub ahead of print] PMID:22544313.
  • Krachler, A-M, Ham, H. & Orth, K. Turnabout is fair play: Use of the bacterial Multivalent Adhesion Molecule 7 as an antimicrobial agent. Virulence (2012) 1;3(1).

2011

  • Krachler AM & Orth, K. Functional characterization of the interaction between bacterial adhesin multivalent adhesion molecule 7 (MAM7) protein and its host cell ligands. J Biol Chem. (2011) Nov 11;286(45):38939-47.
  • Grammel M, Luong P, Orth, K, Hang HC. A chemical reporter for protein AMPylation. J Am Chem Soc. (2011) Nov 2;133(43):17103-5.
  • Krachler, A-M, Woolery, A.R. & Orth, K. Host-pathogen interactions: Manipulation of kinase signaling by bacterial pathogens. (2011) J. Cell Biol 195(7):1083-92.
  • Krachler AM, Ham H, Orth, K. The outer membrane adhesion factor MAM7 initiates host cell binding during infection with Gram-negative pathogens. (2011) Proc Natl Acad Sci USA. Epub June 2011.
  • Ham H., Sreelatha, A. & Orth, K. (2011) Manipulation of Host Membranes by Bacterial Effectors. Nature Reviews Microbiology 9(9):635-46.
  • Broberg, CA, Calder, TJ, & Orth, K. Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes Infect. 2011 Nov;13(12-13):992-1001. Epub 2011 Jul 7.
  • Hao YH, Chuang T, Ball HL, Luong P, Li Y, Flores-Saaib RD, Orth, K. Characterization of a rabbit polyclonal antibody against threonine-AMPylation. (2011) J Biotechnol. 151(3):251-4.
  • Li Y, Al-Eryani R, Yerbrough ML, Orth, K, Ball HL. Characterization of AMPylation on threonine, serine, and tyrosine using mass spectrometry. (2011) J Am Soc Mass Spectrom. 22(4):752-61.
  • Krachler AM, Orth, K. Black Spot, Black Death, Black Pearl: Tales of Bacterial Effectors. Chapter in Avancées et nouvelles technologies en Toxinologie – Advances and new technologies in Toxinology (SFET, ed.). ISSN 1760 – 6004.

2010

  • Broberg CA, Zhang L, Gonzalez H, Laskowski-Arce MA, Orth, K. A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity. (2010) Science. 329(5999):1660-2.
  • Luong P, Kinch LN, Brautigam CA, Grishin NV, Tomchick DR, Orth, K. Kinetic and structural insights into the mechanism of AMPylation by VopS Fic domain. (2010) J Biol Chem. 285(26):20155-63.
  • Broberg CA, Orth, K. Tipping the balance by manipulating post-translational modifications. (2010) Curr Opin Microbiol. 13(1):34-40.
  • Woolery AR, Luong P, Broberg CA, Orth, K. AMPylation: Something Old is New Again. (2010) Front Microbiol. 1:113.

2009

  • Burdette DL, Seemann J, Orth, K. Vibrio VopQ induces PI3-kinase-independent autophagy and antagonizes phagocytosis. (2009) Mol Microbiol. 73(4):639-49.
  • Kinch LN, Yarbrough ML, Orth, K, Grishin NV. Fido, a novel AMPylation domain common to fic, doc, and AvrB. (2009) PLoS One. 4(6):e5818.
  • Yarbrough ML, Orth, K. AMPylation is a new post-translational modification. (2009) Nat Chem Biol. 5(6):378-9.
  • Yarbrough ML, Li Y, Kinch LN, Grishin NV, Ball HL, Orth, K. AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling. (2009) Science. 323(5911):269-72.
  • Burdette DL, Yarbrough ML, Orth, K. Not without a cause: Vibrio parahaemolyticus induces acute autophagy and cell death. (2009) Autophagy. 5(1):100-2.

2008

  • Mukherjee S, Orth, K. Microbiology. A protein pupylation paradigm. (2008) Science. 322(5904):1062-3.
  • Laskowski-Arce MA, Orth, K. Acanthamoeba castellanii promotes the survival of Vibrio parahaemolyticus. (2008) Appl Environ Microbiol. 74(23):7183-8.
  • Burdette L, Yarbrough ML, Orvedahl A, Gilpin CJ, Orth, K. Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis. (2008) Proc Natl Acad Sci USA. 105(34):12497-502.
  • Mukherjee S, Orth, K. in vitro signaling by MAPK and NFkappaB pathways inhibited by Yersinia YopJ. (2008) Methods Enzymol. 438:343-53.
  • Le Negrate G, Faustin B, Welsh K, Loeffler M, Krajewska M, Hasegawa P, Mukherjee S, Orth, K, Krajewski S, Godzik A, Guiney DG, Reed JC. Salmonella secreted factor L deubiquitinase of Salmonella typhimurium inhibits NF-kappaB, suppresses IkappaBalpha ubiquitination and modulates innate immune responses. (2008) J Immunol. 180(7):5045-56.
  • Mukherjee S, Negi VS, Keitany G, Tanaka Y, Orth, K. In vitro activation of the IkappaB kinase complex by human T-cell leukemia virus type-1 Tax. (2008) J Biol Chem. 283(22):15127-33.
  • Hao YH, Wang Y, Burdette D, Mukherjee S, Keitany G, Goldsmith E, Orth, K. Structura requirements for Yersinia YopJ inhibition of MAP kinase pathways. (2008) PLoS One. 3(1):e1375.
  • Trosky JE, Liverman AD, Orth, K. Yersinia outer proteins: Yops. (2008) Cell Microbiol. 10(3):557-65.

2007 & earlier

PubMed