Publications

Organization of Chromatin by Intrinsic and Regulated Phase Separation.

Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK, 2019 Sep Cell

Biomolecular condensates: organizers of cellular biochemistry.

Banani SF, Lee HO, Hyman AA, Rosen MK 2017 Feb Nat. Rev. Mol. Cell Biol.

Compositional Control of Phase-Separated Cellular Bodies.

Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK 2016 Jul Cell 3 166 651-63

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein.

Pak CW, Kosno M, Holehouse AS, Padrick SB, Mittal A, Ali R, Yunus AA, Liu DR, Pappu RV, Rosen MK 2016 Jul Mol. Cell 1 63 72-85

Phase separation of signaling molecules promotes T cell receptor signal transduction.

Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton J, Rosen MK, Vale RD 2016 Apr Science 6285 352 595-9

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins.

Lin Y, Protter DS, Rosen MK, Parker R 2015 Sep Mol. Cell

Phase transitions of multivalent proteins can promote clustering of membrane receptors.

Banjade S, Rosen MK 2014 Oct Elife 3

Phase transitions in the assembly of multivalent signalling proteins.

Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK 2012 Mar Nature 7389 483 336-40

Structural Mechanism of WASP Activation by the Enterohaemorrhagic E. Coli Effector EspFu

Cheng, H.-C., Skehan, B. M., Leong, J. M., Rosen, M. K. May 2008 454 1009-1013

Internal Dynamics Control Activation and Activity of the Autoinhibited Vav DH Domain

Li, P., Martins, I. R. S., Amarasinghe, G. K. and Rosen, M. K. May 2008 15(6) 613-618

ExoSloNano: Multi-Modal Nanogold Tags for identification of Macromolecules in Live Cells & Cryo-Electron Tomograms.

Young LN, Sherrard A, Zhou H, Shaikh F, Hutchings J, Riggi M, Rosen MK, Giraldez AJ, Villa E, 2024 Oct bioRxiv

Surface Charge Can Modulate Phase Separation of Multidomain Proteins.

Kim J, Qin S, Zhou HX, Rosen MK, 2024 Jan J Am Chem Soc

Advanced Surface Passivation for High-Sensitivity Studies of Biomolecular Condensates.

Yao RW, Rosen MK, 2024 Feb bioRxiv

Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct.

Kosno M, Currie SL, Kumar A, Xing C, Rosen MK, 2023 May bioRxiv

In diverse conditions, intrinsic chromatin condensates have liquid-like material properties.

Gibson BA, Blaukopf C, Lou T, Chen L, Doolittle LK, Finkelstein I, Narlikar GJ, Gerlich DW, Rosen MK, 2023 May Proc Natl Acad Sci U S A 18 120 e2218085120

Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct.

Kosno M, Currie SL, Kumar A, Xing C, Rosen MK, 2023 Jul Sci Rep 1 13 11907

Quantitative reconstitution of yeast RNA processing bodies.

Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK, 2023 Apr Proc Natl Acad Sci U S A 14 120 e2214064120

Coupling of protein condensates to ordered lipid domains determines functional membrane organization.

Wang HY, Chan SH, Dey S, Castello-Serrano I, Rosen MK, Ditlev JA, Levental KR, Levental I, 2023 Apr Sci Adv 17 9 eadf6205

A mitotic chromatin phase transition prevents perforation by microtubules.

Schneider MWG, Gibson BA, Otsuka S, Spicer MFD, Petrovic M, Blaukopf C, Langer CCH, Batty P, Nagaraju T, Doolittle LK, Rosen MK, Gerlich DW, 2022 Sep Nature 7925 609 183-190

Bound nucleotide can control the dynamic architecture of monomeric actin.

Ali R, Zahm JA, Rosen MK, 2022 Mar Nat Struct Mol Biol

Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation.

Case LB, De Pasquale M, Henry L, Rosen MK, 2022 Jan Elife 11

Poly-glutamine-dependent self-association as a potential mechanism for regulation of androgen receptor activity.

Roggero CM, Esser V, Duan L, Rice AM, Ma S, Raj GV, Rosen MK, Liu ZP, Rizo J, 2022 PLoS One 1 17 e0258876

Small Molecule Properties Define Partitioning into Biomolecular Condensates

Sabareesan Ambadi Thody, Hanna D. Clements, Hamid Baniasadi, Andrew S. Lyon, Matthew S. Sigman, Michael K. Rosen 2022 bioRxiv 2022.12.19.521099

Beth Levine M.D. Prize in Autophagy Research.

Vitetta ES, Cobb MH, Hobbs HH, Hooper L, Morrison SJ, Orth K, Pfeiffer J, Rosen MK, Takahashi JS, Wang T, 2021 Sep Autophagy 9 17 2053

Using quantitative reconstitution to investigate multi-component condensates.

Currie SL, Rosen MK, 2021 Nov RNA

The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains.

Zhemkov V, Ditlev JA, Lee WR, Wilson M, Liou J, Rosen MK, Bezprozvanny I, 2021 May Elife 10

Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin.

Strohkendl I, Saifuddin FA, Gibson BA, Rosen MK, Russell R, Finkelstein IJ, 2021 Mar Sci Adv 11 7

Mechanistic dissection of increased enzymatic rate in a phase-separated compartment.

Peeples W, Rosen MK, 2021 Jun Nat Chem Biol 6 17 693-702

Structure-Function Properties in Disordered Condensates.

Bhandari K, Cotten MA, Kim J, Rosen MK, Schmit JD, 2021 Jan J Phys Chem B 1 125 467-476

Beth Levine M.D. Prize in Autophagy Research.

Vitetta ES, Cobb MH, Hobbs HH, Hooper L, Morrison SJ, Orth K, Pfeiffer J, Rosen MK, Takahashi JS, Wang T, 2021 Aug Autophagy 1

A framework for understanding the functions of biomolecular condensates across scales.

Lyon AS, Peeples WB, Rosen MK, 2020 Nov Nat Rev Mol Cell Biol

Karyopherins and condensates.

Springhower CE, Rosen MK, Chook YM, 2020 May Curr. Opin. Cell Biol. 64 112-123

Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein.

Zhang R, Lee DM, Jimah JR, Gerassimov N, Yang C, Kim S, Luvsanjav D, Winkelman J, Mettlen M, Abrams ME, Kalia R, Keene P, Pandey P, Ravaux B, Kim JH, Ditlev JA, Zhang G, Rosen MK, Frost A, Alto NM, Gardel M, Schmid SL, Svitkina TM, Hinshaw JE, Chen EH, 2020 May Nat. Cell Biol.

A quantitative inventory of yeast P body proteins reveals principles of composition and specificity.

Xing W, Muhlrad D, Parker R, Rosen MK, 2020 Jun Elife 9

Improved strategy for isoleucine 1H/13C methyl labeling in Pichia pastoris.

Ali R, Clark LD, Zahm JA, Lemoff A, Ramesh K, Rosenbaum DM, Rosen MK, 2019 Sep J. Biomol. NMR

Phosphorylation of Nephrin induces phase separated domains that move through actomyosin contraction.

Kim S, Kalappurakkal JM, Mayor S, Rosen MK, 2019 Oct Mol. Biol. Cell mbcE18120823

A composition-dependent molecular clutch between T cell signaling condensates and actin.

Ditlev JA, Vega AR, Köster DV, Su X, Tani T, Lakoduk AM, Vale RD, Mayor S, Jaqaman K, Rosen MK, 2019 Jul Elife 8

Regulation of Transmembrane Signaling by Phase Separation.

Case LB, Ditlev JA, Rosen MK, 2019 Apr Annu Rev Biophys

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins.

Case LB, Zhang X, Ditlev JA, Rosen MK, 2019 03 Science 6431 363 1093-1097

Intrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly.

Protter DSW, Rao BS, Van Treeck B, Lin Y, Mizoue L, Rosen MK, Parker R 2018 Feb Cell Rep 6 22 1401-1412

Who's in and Who's Out-Compositional Control of Biomolecular Condensates.

Ditlev JA, Case LB, Rosen MK 2018 Aug J. Mol. Biol.

Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites.

Yoshizawa T, Ali R, Jiou J, Fung HYJ, Burke KA, Kim SJ, Lin Y, Peeples WB, Saltzberg D, Soniat M, Baumhardt JM, Oldenbourg R, Sali A, Fawzi NL, Rosen MK, Chook YM 2018 Apr Cell 3 173 693-705.e22

Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites.

Chen B, Chou HT, Brautigam CA, Xing W, Yang S, Henry L, Doolittle LK, Walz T, Rosen MK 2017 Sep Elife 6

Intrinsically disordered sequences enable modulation of protein phase separation through distributed tyrosine motifs.

Lin Y, Currie SL, Rosen MK 2017 Sep J. Biol. Chem.

Allosteric Modulation of Grb2 Recruitment to the Intrinsically Disordered Scaffold Protein, LAT, by Remote Site Phosphorylation.

Huang WYC, Ditlev JA, Chiang HK, Rosen MK, Groves JT 2017 Nov J. Am. Chem. Soc.

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins.

Harmon TS, Holehouse AS, Rosen MK, Pappu RV 2017 Nov Elife 6

ATP controls the crowd.

Rice AM, Rosen MK 2017 May Science 6339 356 701-702

Reconstitution of TCR Signaling Using Supported Lipid Bilayers.

Su X, Ditlev JA, Rosen MK, Vale RD 2017 Methods Mol. Biol. 1584 65-76

Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury in Drosophila melanogaster.

Srinivasan N, Gordon O, Ahrens S, Franz A, Deddouche S, Chakravarty P, Phillips D, Yunus AA, Rosen MK, Valente RS, Teixeira L, Thompson B, Dionne MS, Wood W, Reis E Sousa C 2016 Nov Elife 5

Structural and mechanistic insights into regulation of the retromer coat by TBC1d5.

Jia D, Zhang JS, Li F, Wang J, Deng Z, White MA, Osborne DG, Phillips-Krawczak C, Gomez TS, Li H, Singla A, Burstein E, Billadeau DD, Rosen MK 2016 Nov Nat Commun 7 13305

Correction: Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation.

Squarr AJ, Brinkmann K, Chen B, Steinbacher T, Ebnet K, Rosen MK, Bogdan S 2016 Mar J. Cell Biol. 7 212 883

Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation.

Squarr AJ, Brinkmann K, Chen B, Steinbacher T, Ebnet K, Rosen MK, Bogdan S 2016 Feb J. Cell Biol. 5 212 591-603

Erratum to: Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris.

Clark L, Zahm JA, Ali R, Kukula M, Bian L, Patrie SM, Gardner KH, Rosen MK, Rosenbaum DM 2016 Feb J. Biomol. NMR

Data publication with the structural biology data grid supports live analysis.

Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Christopher Fromme J, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz P 2016 Nat Commun 7 10882

Conserved interdomain linker promotes phase separation of the multivalent adaptor protein Nck.

Banjade S, Wu Q, Mittal A, Peeples WB, Pappu RV, Rosen MK 2015 Nov Proc. Natl. Acad. Sci. U.S.A.

Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris.

Clark L, Zahm JA, Ali R, Kukula M, Bian L, Patrie SM, Gardner KH, Rosen MK, Rosenbaum DM 2015 May J. Biomol. NMR

Ena/VASP Proteins Cooperate with the WAVE Complex to Regulate the Actin Cytoskeleton.

Chen XJ, Squarr AJ, Stephan R, Chen B, Higgins TE, Barry DJ, Martin MC, Rosen MK, Bogdan S, Way M 2014 Sep Dev. Cell 5 30 569-84

Retromer Binding to FAM21 and the WASH Complex Is Perturbed by the Parkinson Disease-Linked VPS35(D620N) Mutation.

McGough IJ, Steinberg F, Jia D, Barbuti PA, McMillan KJ, Heesom KJ, Whone AL, Caldwell MA, Billadeau DD, Rosen MK, Cullen PJ 2014 Jul Curr. Biol. 14 24 1670-6

Retromer Binding to FAM21 and the WASH Complex Is Perturbed by the Parkinson Disease-Linked VPS35(D620N) Mutation.

McGough IJ, Steinberg F, Jia D, Barbuti PA, McMillan KJ, Heesom KJ, Whone AL, Caldwell MA, Billadeau DD, Rosen MK, Cullen PJ 2014 Jul Curr. Biol. 14 24 1678

Local F-actin Network Links Synapse Formation and Axon Branching.

Chia PH, Chen B, Li P, Rosen MK, Shen K 2014 Jan Cell 1-2 156 208-20

The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton.

Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MK 2014 Jan Cell 1-2 156 195-207

Biochemical reconstitution of the WAVE regulatory complex.

Chen B, Padrick SB, Henry L, Rosen MK 2014 Meth. Enzymol. 540 55-72

The Haemophilus ducreyi LspA1 Protein Inhibits Phagocytosis By Using a New Mechanism Involving Activation of C-Terminal Src Kinase.

Dodd DA, Worth RG, Rosen MK, Grinstein S, van Oers NS, Hansen EJ 2014 MBio 3 5

The bacterial effector VopL organizes actin into filament-like structures.

Zahm JA, Padrick SB, Chen Z, Pak CW, Yunus AA, Henry L, Tomchick DR, Chen Z, Rosen MK 2013 Oct Cell 2 155 423-34

Regulation of WASH-Dependent Actin Polymerization and Protein Trafficking by Ubiquitination.

Hao YH, Doyle JM, Ramanathan S, Gomez TS, Jia D, Xu M, Chen ZJ, Billadeau DD, Rosen MK, Potts PR 2013 Feb Cell 5 152 1051-64

Measurement and analysis of in vitro actin polymerization.

Doolittle LK, Rosen MK, Padrick SB 2013 Methods Mol. Biol. 1046 273-93

Purification of Arp2/3 Complex from Saccharomyces cerevisiae.

Doolittle LK, Rosen MK, Padrick SB 2013 Methods Mol. Biol. 1046 251-71

Purification of native arp2/3 complex from bovine thymus.

Doolittle LK, Rosen MK, Padrick SB 2013 Methods Mol. Biol. 1046 231-50

Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation.

Smith BA, Padrick SB, Doolittle LK, Daugherty-Clarke K, Corrêa IR, Xu MQ, Goode BL, Rosen MK, Gelles J 2013 Elife 2 e01008

Enlightening molecular mechanisms through study of protein interactions.

Rizo J, Rosen MK, Gardner KH 2012 Oct J Mol Cell Biol 5 4 270-83

Multiple repeat elements within the FAM21 tail link the WASH actin regulatory complex to the retromer.

Jia D, Gomez TS, Billadeau DD, Rosen MK 2012 Jun Mol. Biol. Cell 12 23 2352-61

Mechanism of actin filament nucleation by the bacterial effector VopL.

Yu B, Cheng HC, Brautigam CA, Tomchick DR, Rosen MK 2011 Sep Nat. Struct. Mol. Biol. 9 18

The feasibility of parameterizing four-state equilibria using relaxation dispersion measurements.

Li P, Martins IR, Rosen MK 2011 Sep J. Biomol. NMR 1-2 51 57-70

Structure and control of the actin regulatory WAVE complex.

Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK 2010 Nov Nature 7323 468 533-8

WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes.

Jia D, Gomez TS, Metlagel Z, Umetani J, Otwinowski Z, Rosen MK, Billadeau DD 2010 Jun Proc. Natl. Acad. Sci. U.S.A. 23 107 10442-7

Activating WASP mutations associated with X-linked neutropenia result in enhanced actin polymerization, altered cytoskeletal responses, and genomic instability in lymphocytes.

Westerberg LS, Meelu P, Baptista M, Eston MA, Adamovich DA, Cotta-de-Almeida V, Seed B, Rosen MK, Vandenberghe P, Thrasher AJ, Klein C, Alt FW, Snapper SB 2010 Jun J. Exp. Med. 6 207 1145-52

Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1.

Yu B, Martins IR, Li P, Amarasinghe GK, Umetani J, Fernandez-Zapico ME, Billadeau DD, Machius M, Tomchick DR, Rosen MK 2010 Jan Cell 2 140 246-56

Determination of protein complex stoichiometry through multisignal sedimentation velocity experiments.

Padrick SB, Deka RK, Chuang JL, Wynn RM, Chuang DT, Norgard MV, Rosen MK, Brautigam CA 2010 Dec Anal. Biochem. 1 407 89-103

Rationally improving LOV domain-based photoswitches.

Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR 2010 Aug Nat. Methods 8 7 623-6

Physical mechanisms of signal integration by WASP family proteins.

Padrick SB, Rosen MK 2010 Annu. Rev. Biochem. 79 707-35

Crystal structure of the Formin mDia1 in autoinhibited conformation.

Otomo T, Tomchick DR, Otomo C, Machius M, Rosen MK 2010 PLoS ONE 9 5

Structural and Energetic Mechanisms of Cooperative Autoinhibition and Activation of Vav1

Yu, B., Martins, I. R. S., Li, P., Amarasinghe, G. K., Umetani, J., Fernandez-Zapico, M., Billadeau, D. D., Machius, M., Tomchick, D. R., Rosen, M. K. 2010 140 246-256

The WAVE regulatory complex is inhibited.

Ismail AM, Padrick SB, Chen B, Umetani J, Rosen MK 2009 May Nat. Struct. Mol. Biol. 5 16 561-3

A large kindred with X-linked neutropenia with an I294T mutation of the Wiskott-Aldrich syndrome gene.

Beel K, Cotter MM, Blatny J, Bond J, Lucas G, Green F, Vanduppen V, Leung DW, Rooney S, Smith OP, Rosen MK, Vandenberghe P 2009 Jan Br. J. Haematol. 1 144 120-6

Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway.

Leung DW, Otomo C, Chory J, Rosen MK 2008 Sep Proc. Natl. Acad. Sci. U.S.A. 35 105 12797-802

Repetitive N-WASP-binding elements of the enterohemorrhagic Escherichia coli effector EspF(U) synergistically activate actin assembly.

Campellone KG, Cheng HC, Robbins D, Siripala AD, McGhie EJ, Hayward RD, Welch MD, Rosen MK, Koronakis V, Leong JM 2008 Oct PLoS Pathog. 10 4 e1000191

Hierarchical regulation of WASP/WAVE proteins.

Padrick SB, Cheng HC, Ismail AM, Panchal SC, Doolittle LK, Kim S, Skehan BM, Umetani J, Brautigam CA, Leong JM, Rosen MK 2008 Nov Mol. Cell 3 32 426-38

Internal dynamics control activation and activity of the autoinhibited Vav DH domain.

Li P, Martins IR, Amarasinghe GK, Rosen MK 2008 Jun Nat. Struct. Mol. Biol. 6 15 613-8

Estimation of the available free energy in a LOV2-J alpha photoswitch.

Yao X, Rosen MK, Gardner KH 2008 Aug Nat. Chem. Biol. 8 4 491-7

Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U).

Cheng HC, Skehan BM, Campellone KG, Leong JM, Rosen MK 2008 Aug Nature 7207 454 1009-13

Hierarchical Regulation of WASP/WAVE Proteins

Padrick, S. B., Cheng, H.-C. C., Ismail, A., Panchal, S. C., Doolittle, L. K., Kim, S., Skehan, B.M., Umetani, J., Brautigam, C. A., Leong, J. M., Rosen, M. K. 2008 32 426-438

Arp2/3-independent assembly of actin by Vibrio type III effector VopL.

Liverman AD, Cheng HC, Trosky JE, Leung DW, Yarbrough ML, Burdette DL, Rosen MK, Orth K 2007 Oct Proc. Natl. Acad. Sci. U.S.A. 43 104 17117-22

Signal transduction pathway of TonB-dependent transporters.

Ferguson AD, Amezcua CA, Halabi NM, Chelliah Y, Rosen MK, Ranganathan R, Deisenhofer J 2007 Jan Proc. Natl. Acad. Sci. U.S.A. 2 104 513-8

Protein-tyrosine kinase and GTPase signals cooperate to phosphorylate and activate Wiskott-Aldrich syndrome protein (WASP)/neuronal WASP.

Torres E, Rosen MK 2006 Feb J. Biol. Chem. 6 281 3513-20

Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLalpha and mDia1.

Seth A, Otomo C, Rosen MK 2006 Aug J. Cell Biol. 5 174 701-13

Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization.

Tskvitaria-Fuller I, Seth A, Mistry N, Gu H, Rosen MK, Wülfing C 2006 Aug J. Immunol. 3 177 1708-20

Biochemical properties and inhibitors of (N-)WASP.

Leung DW, Morgan DM, Rosen MK 2006 Meth. Enzymol. 406 281-96

A novel mechanism of actin filament processive capping by formin: solution of the rotation paradox.

Shemesh T, Otomo T, Rosen MK, Bershadsky AD, Kozlov MM 2005 Sep J. Cell Biol. 6 170 889-93

Acidic region tyrosines provide access points for allosteric activation of the autoinhibited Vav1 Dbl homology domain.

Amarasinghe GK, Rosen MK 2005 Nov Biochemistry 46 44 15257-68

NMR analyses of the activation of the Arp2/3 complex by neuronal Wiskott-Aldrich syndrome protein.

Kreishman-Deitrick M, Goley ED, Burdine L, Denison C, Egile C, Li R, Murali N, Kodadek TJ, Welch MD, Rosen MK 2005 Nov Biochemistry 46 44 15247-56

Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain.

Otomo T, Tomchick DR, Otomo C, Panchal SC, Machius M, Rosen MK 2005 Feb Nature 7025 433 488-94

[Structure and function of Formin homology 2 domain].

Otomo T, Rosen MK 2005 Aug Tanpakushitsu Kakusan Koso 9 50 1088-93

Structural basis of Rho GTPase-mediated activation of the formin mDia1.

Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK 2005 Apr Mol. Cell 3 18 273-81

The nucleotide switch in Cdc42 modulates coupling between the GTPase-binding and allosteric equilibria of Wiskott-Aldrich syndrome protein.

Leung DW, Rosen MK 2005 Apr Proc. Natl. Acad. Sci. U.S.A. 16 102 5685-90

Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation.

Peterson JR, Bickford LC, Morgan D, Kim AS, Ouerfelli O, Kirschner MW, Rosen MK 2004 Aug Nat. Struct. Mol. Biol. 8 11 747-55

A two-state allosteric model for autoinhibition rationalizes WASP signal integration and targeting.

Buck M, Xu W, Rosen MK 2004 Apr J. Mol. Biol. 2 338 271-85

Itk functions to control actin polymerization at the immune synapse through localized activation of Cdc42 and WASP.

Labno CM, Lewis CM, You D, Leung DW, Takesono A, Kamberos N, Seth A, Finkelstein LD, Rosen MK, Schwartzberg PL, Burkhardt JK 2003 Sep Curr. Biol. 18 13 1619-24

Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP.

Torres E, Rosen MK 2003 May Mol. Cell 5 11 1215-27

Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6.

Garrard SM, Capaldo CT, Gao L, Rosen MK, Macara IG, Tomchick DR 2003 Mar EMBO J. 5 22 1125-33

NMR analysis of methyl groups at 100-500 kDa: model systems and Arp2/3 complex.

Kreishman-Deitrick M, Egile C, Hoyt DW, Ford JJ, Li R, Rosen MK 2003 Jul Biochemistry 28 42 8579-86

A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex.

Panchal SC, Kaiser DA, Torres E, Pollard TD, Rosen MK 2003 Aug Nat. Struct. Biol. 8 10 591-8

Rational design of genetically encoded fluorescence resonance energy transfer-based sensors of cellular Cdc42 signaling.

Seth A, Otomo T, Yin HL, Rosen MK 2003 Apr Biochemistry 14 42 3997-4008

Uncoupling Kapbeta2 substrate dissociation and ran binding.

Chook YM, Jung A, Rosen MK, Blobel G 2002 Jun Biochemistry 22 41 6955-66

Ignition of a cellular machine.

Kreishman-Deitrick M, Rosen MK, Kreishman-Deltrick M 2002 Feb Nat. Cell Biol. 2 4 E31-3

Retinopathies associated with antiretinal antibodies.

Hooks JJ, Tso MO, Detrick B, 2001 Sep Clin Diagn Lab Immunol 5 8 853-8

Structural biology. Flipping a switch.

Buck M, Rosen MK 2001 Mar Science 5512 291 2329-30

Global folds of highly deuterated, methyl-protonated proteins by multidimensional NMR.

Gardner KH, Rosen MK, Kay LE 1997 Feb Biochemistry 6 36 1389-401

An (H)C(CO)NH-TOCSY pulse scheme for sequential assignment of protonated methyl groups in otherwise deuterated (15)N, (13)C-labeled proteins.

Gardner KH, Konrat R, Rosen MK, Kay LE 1996 Oct J. Biomol. NMR 3 8 351-6

Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein.

Rosen MK, Yamazaki T, Gish GD, Kay CM, Pawson T, Kay LE 1995 Mar Nature 6521 374 477-9

Comparison of six strains of Trichomonas foetus. II. Gel-diffusion precipitin studies of soluble antigens.

Johnson AE 1967 Dec Exp. Parasitol. 3 21 336-43