Our lab works with murine disease models and employs Biochemistry, Molecular and Cell Biology to investigate brain glycogen metabolism and related neurodegenerative diseases.

My lab has a long-time interest in understanding the mechanisms of transcription and gene regulation in mammalian cells using initially cell-free systems reconstituted with purified gene-specific transcription factors, general cofactors, and components of the general transcription machinery to recapitulate transcriptional events in vitro. 

We focus on the discovery of targeted therapies for major drivers of cancer using protein chemistry, enzymology, structural biology, informatics and cell biology. Some of our favorite targets are RAS and kinase proteins.

We are developing inhibitors of pyrimidine biosynthesis and polyamine biosynthesis to treat malaria and African sleeping sickness. We study polyamine and nucleotide metabolism in African trypanosomes to learn about novel metabolism and regulation.

The Kohler research group is committed to developing and implementing new tools optimized for the study of glycosylated molecules.